Continuous production of galacto-oligosaccharides from lactose by Bullera singularis β-galactosidase immobilized in chitosan beads

被引：77

作者：

Shin, HJ ^{[1
]}

Park, JM

Yang, JW

机构：

[1] KAIST, Dept Chem Engn, Taejon 302701, South Korea

[2] POSTECH, Dept Chem Engn, Pohang 790600, South Korea

来源：

PROCESS BIOCHEMISTRY | 1998年 / 33卷 / 08期

关键词：

galacto-oligosaccharide (GalOS); Bullera singularis; continuous production; immobilization; chitosan bead; lactose hydrolysis;

D O I：

10.1016/S0032-9592(98)00045-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Galacto-oligosaccharides (GalOS) were continuously produced using lactose and immobilized beta-galactosidase from Bullera singularis ATCC 24193 in a packed bed reactor. Partially purified beta-galactosidase was immobilized in Chitopearl BCW 3510 bead (970 GU/g resin) by simple adsorption. 55% (w/w) oligosaccharides was obtained continuously with a productivity of 4.4 g/(litre-h) from 100 g/litre lactose solution during a 15-day operation. Batch productivity was 6.5 g GalOS/(litre-h) from 300 g/litre lactose. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：787 / 792

页数：6

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