OLIGOSACCHARIDES SYNTHESIS BY FREE AND IMMOBILIZED BETA-GALACTOSIDASES FROM THERMUS-AQUATICUS YT-1

被引：34

作者：

BERGER, JL

LEE, BH

LACROIX, C

机构：

[1] MCGILL UNIV,DEPT FOOD SCI,ST ANNE BELLEVUE,PQ H9X 3V9,CANADA

[2] AGR CANADA,CTR FOOD RES & DEV,ST HYACINTHE,PQ J2S 8E3,CANADA

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 10期

关键词：

D O I：

10.1007/BF00143104

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Galactooligosaccharides (GalOS) production from a lactose solution (16% w/v) was studied at 70 degrees C, pH 4.6 and 6.0 with free or immobilized beta-galactosidases of Thermus aquaticus YT-1. Synthesis of OS and lactose conversion was significantly improved when using the immobilized preparation. At pH 4.6, OS accounted for approx. 35% of the total carbohydrates with a conversion rate of lactose of 80%. The general formula of OS synthesized in this way was Gal-(Gal)n-Glu with n being equal to 1 or 2 and a beta anomeric configuration of the D-galactosyl moiety.

引用

页码：1077 / 1080

页数：4

共 13 条

[1] IMMOBILIZATION OF BETA-GALACTOSIDASES FROM THERMUS-AQUATICUS YT-1 FOR OLIGOSACCHARIDES SYNTHESIS [J].