IMMOBILIZATION OF BETA-GALACTOSIDASES FROM THERMUS-AQUATICUS YT-1 FOR OLIGOSACCHARIDES SYNTHESIS

beta-galactosidases of Thermus aquaticus YT-1, exhibiting a galactosyl transferase activity, were immobilized using different techniques. Entrapment in agarose or gellan gum beads was unsuitable for enzyme immobilization due to enzyme leakage. A technique that efficiently immobilized the enzymes was developed using glutaraldehyde co-crosslinking of beta-galactosidases with bovine serum albumin, followed by entrapment in agarose beads.