Specificity of PCR and Serological Assays in the Detection of Escherichia coli Shiga Toxin Subtypes

被引：65

作者：

Feng, Peter C. H. ^{[1
]}

Jinneman, Karen ^{[2
]}

Scheutz, Flemming ^{[3
]}

Monday, Steven R. ^{[1
]}

机构：

[1] US FDA, Div Microbiol, College Pk, MD 20740 USA

[2] US FDA, Pacific Reg Lab NW, Bothell, WA USA

[3] WHO Collaborating Ctr Reference & Res Escherichia, Copenhagen, Denmark

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 18期

关键词：

VARIANTS; STRAINS; IDENTIFICATION; ASSOCIATION; O157-H7; STEC; GENE; FOOD;

D O I：

10.1128/AEM.00370-11

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Specificity analysis for stx or Stx subtypes in Escherichia coli showed that the PCR assays we tested did not detect stx(1d) and stx(2f), and some also missed stx(2b) and stx(2g). Most of the serological assays examined did not detect Stx2c, Stx2e, Stx2f, and Stx2g, and some strain-to-strain variation in reactivity was observed for Stx2b.

引用

页码：6699 / 6702

页数：4

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