Activation of human Vα24NKT cells by α-glycosylceramide in a CD1d-restricted and Vα24TCR-mediated manner

被引：90

作者：

Nieda, M

Nicol, A

Koezuka, Y

Kikuchi, A

Takahashi, T

Nakamura, H

Furukawa, H

Yabe, T

Ishikawa, Y

Tadokoro, K

Juji, T

机构：

[1] Japanese Red Cent Blood Ctr, Dept Res, Shibuya Ku, Tokyo, Japan

[2] Royal Brisbane Hosp, Bone Marrow Transplant Unit, Herston, Qld 4029, Australia

[3] Kirin Brewery Co Ltd, Pharmaceut Res Lab, Takasaki, Gumma 37012, Japan

[4] Univ Tokyo, Fac Med, Bunkyo Ku, Tokyo 113, Japan

来源：

HUMAN IMMUNOLOGY | 1999年 / 60卷 / 01期

关键词：

V alpha 24NKT; NKR-P1A; alpha-glycosylceramide; CD1d molecules; dendritic cells;

D O I：

10.1016/S0198-8859(98)00100-1

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

V alpha 14NK(natural killer) T cells play an important role in controlling tumors or in preventing autoimmunity in the murine system. V alpha 24NKT cells, the human counterpart of V alpha 14NKT cells, may contribute to controlling the progression of autoimmune diseases in humans. These findings show the possibility that ligand(s) for these NKT cells can control the above-mentioned pathological conditions. Specific glycolipids such as alpha-galactosylceramide (alpha-GalCer) and alpha-glucosylceramide (alpha-GlcCer) have been identified as ligand(s) recognized by murine V alpha 14NKT cells in a CD1d-restricted manner, but it remains unclear whether these glycolipids are ligand(s) for Va24NKT cells in humans. To determine whether alpha-glycosylceramide is presented by CD1d molecules in humans, we initially established a V alpha 24NKT cell line specific for alpha-glycosylceramide using dendritic cell (DC) like cells from normal peripheral blood mononuclear cells (PBMC) in an autologous mixed leukocyte reaction (auto-MLR) system, and characterized the V alpha 24NKT cell line. The Va24NKT cells were CD3+ CD4-CD8-V alpha 24+V beta 11+NKRP1A+ and specifically proliferated in response to alpha-glycosylceramide in CD1d-restricted and V alpha 24TCR-mediated manner, The phenotypic and functional similarities between murine V alpha 14NKT cells and human V alpha 24NKT cells suggest that V alpha 24NKT cells may play an important role in controlling tumors or in preventing autoimmunity as observed which V alpha 14NKT cells. (C) American Society for Histocompatibility and Immunogenetics, 1999. Published by Elsevier Science Inc.

引用

页码：10 / 19

页数：10

共 47 条

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