Covalently incorporated protein-nanogels using AGET ATRP in an inverse miniemulsion

被引：60

作者：

Averick, Saadyah E. ^{[1
]}

Magenau, Andrew J. D. ^{[1
]}

Simakova, Antonina ^{[1
]}

Woodman, Bradley F. ^{[2
]}

Seong, Andrew ^{[1
]}

Mehl, Ryan A. ^{[2
]}

Matyjaszewski, Krzysztof ^{[1
]}

机构：

[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[2] Franklin & Marshall Coll, Dept Chem, Lancaster, PA 17604 USA

来源：

POLYMER CHEMISTRY | 2011年 / 2卷 / 07期

基金：

美国国家科学基金会; 美国安德鲁·梅隆基金会;

关键词：

TRANSFER RADICAL POLYMERIZATION; DELIVERY CARRIERS; BIODEGRADATION; PEGYLATION; ATTACHMENT; POLYMERS; LIPASE; GROWTH; ENZYME;

D O I：

10.1039/c1py00050k

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Using a genetically engineered protein, containing a non-natural amino acid bearing an atom transfer radical polymerization (ATRP) initiator, protein-nanogel hybrids (PNHs) were synthesized by activator generated by electron transfer (AGET) ATRP in an inverse miniemulsion. The route presented is an appropriate synthetic strategy to covalently and site specifically incorporate green fluorescent protein (GFP) into well-defined nanogels. These PNHs were analyzed using dynamic light scattering (DLS), UV-visible fluorescence spectroscopy and confocal microscopy to confirm the successful integration of GFP proteins into each nanogel (NG), while preserving its native tertiary structure.

引用

页码：1476 / 1478

页数：3

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