Glycerol diversifies phage repertoire selections and lowers non-specific phage absorption

被引：11

作者：

Kjær, S ^{[1
]}

Stausbol-Gron, B ^{[1
]}

Wind, T ^{[1
]}

Ravn, P ^{[1
]}

Jensen, KP ^{[1
]}

Kahns, L ^{[1
]}

Clark, BFC ^{[1
]}

机构：

[1] Aarhus Univ, Inst Mol & Struct Biol, DK-8000 Aarhus, Denmark

来源：

FEBS LETTERS | 1998年 / 431卷 / 03期

关键词：

phage antibody; selection strategy; glycerol; elongation factor 1A; dominant clone;

D O I：

10.1016/S0014-5793(98)00789-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a semi-synthetic phage displayed antibody repertoire, isoform-specific and cross-reactive phage-antibodies to eukaryotic elongation factor 1A (eEF1A) have been selected. Enrichment of specific antibodies was found to depend on the presence of glycerol, Further selections against lactate dehydrogenase (LDH) revealed that the dominance of a phage-antibody clone to LDH was inhibited by glycerol, a notable feature for selection strategies where a broad variety of binding clones is desired. The impact of glycerol in distinct steps of the selection protocol was examined and glycerol found to affect certain antibody-antigen interactions. Furthermore, the nonspecific phage binding was lowered by three orders of magnitude at a 20% (v/v) glycerol concentration. (C) 1998 Federation of European Biochemical Societies.

引用

页码：448 / 452

页数：5

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