Strategies for selection of antibodies by phage display

被引：215

作者：

Griffiths, AD

Duncan, AR

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Cambridge Antibody Technol Ltd, Melbourn SG8 6JJ, Cambs, England

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1998年 / 9卷 / 01期

关键词：

D O I：

10.1016/S0958-1669(98)80092-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage antibody-display is rapidly maturing into a very effective tool for antibody generation. The recent development of large primary antibody libraries enables selection of antibodies against most targets in under two weeks and many of these antibodies have relatively high (nanomolar) affinities. Successful strategies have also been developed to affinity mature these antibodies into the picomolar range if required. (C) Current Biology Ltd ISSN 0958-1669.

引用

收藏

页码：102 / 108

页数：7

相关论文

共 64 条

[1] ANTIBODY ENGINEERING BY PARSIMONIOUS MUTAGENESIS [J].

BALINT, RF ;

LARRICK, JW .

GENE, 1993, 137 (01) :109-118

[2] Selection and evolution of high-affinity human anti-viral antibodies [J].

Barbas, CF ;

Burton, DR .

TRENDS IN BIOTECHNOLOGY, 1996, 14 (07) :230-234

[3] IN-VITRO EVOLUTION OF A NEUTRALIZING HUMAN-ANTIBODY TO HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 TO ENHANCE AFFINITY AND BROADEN STRAIN CROSS-REACTIVITY [J].

BARBAS, CF ;

HU, D ;

DUNLOP, N ;

SAWYER, L ;

CABABA, D ;

HENDRY, RM ;

NARA, PL ;

BURTON, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (09) :3809-3813

[4] ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE [J].

BARBAS, CF ;

KANG, AS ;

LERNER, RA ;

BENKOVIC, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :7978-7982

[5] A SURFACE EXPRESSION VECTOR FOR ANTIBODY SCREENING [J].

BREITLING, F ;

DUBEL, S ;

SEEHAUS, T ;

KLEWINGHAUS, I ;

LITTLE, M .

GENE, 1991, 104 (02) :147-153

[6] A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS [J].

BURTON, DR ;

BARBAS, CF ;

PERSSON, MAA ;

KOENIG, S ;

CHANOCK, RM ;

LERNER, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (22) :10134-10137

[7]

CHANG CN, 1991, J IMMUNOL, V147, P3610

[8] MAKING ANTIBODY FRAGMENTS USING PHAGE DISPLAY LIBRARIES [J].

CLACKSON, T ;

HOOGENBOOM, HR ;

GRIFFITHS, AD ;

WINTER, G .

NATURE, 1991, 352 (6336) :624-628

[9] IN-VITRO SELECTION FROM PROTEIN AND PEPTIDE LIBRARIES [J].

CLACKSON, T ;

WELLS, JA .

TRENDS IN BIOTECHNOLOGY, 1994, 12 (05) :173-184

[10] SELECTION OF SPECIFIC PHAGE-DISPLAY ANTIBODIES USING LIBRARIES DERIVED FROM CHICKEN IMMUNOGLOBULIN GENES [J].

DAVIES, EL ;

SMITH, JS ;

BIRKETT, CR ;

MANSER, JM ;

ANDERSONDEAR, DV ;

YOUNG, JR .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 186 (01) :125-135

← 1 2 3 4 5 6 7 →