学术探索
学术期刊
新闻热点
数据分析
智能评审

A LARGE ARRAY OF HUMAN MONOCLONAL-ANTIBODIES TO TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS FROM COMBINATORIAL LIBRARIES OF ASYMPTOMATIC SEROPOSITIVE INDIVIDUALS

被引：584
作者：
BURTON, DR
BARBAS, CF
PERSSON, MAA
KOENIG, S
CHANOCK, RM
LERNER, RA
机构：
[1] SCRIPPS RES INST, DEPT CHEM, LA JOLLA, CA 92037 USA
[2] NIAID, INFECT DIS LAB, BETHESDA, MD 20892 USA
[3] UNIV SHEFFIELD, KREBS INST, DEPT MOLEC BIOL & BIOTECHNOL, SHEFFIELD S10 2TN, S YORKSHIRE, ENGLAND
[4] KAROLINSKA INST, KARALINSKA HOSP, DEPT MED, S-10401 STOCKHOLM 60, SWEDEN
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 22期
关键词：
AIDS; ANTIBODY REPERTOIRES; PASSIVE IMMUNIZATION; FILAMENTOUS PHAGE; PHAGE SURFACE EXPRESSION;
D O I：
10.1073/pnas.88.22.10134
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A panel of human monoclonal antibody Fab fragments has been generated against the surface glycoprotein gp120 of type 1 human immunodeficiency virus (HIV) by antigen selection from a random combinatorial library expressed on the surface of filamentous phage. The library was prepared from 5 ml of bone marrow from an asymptomatic individual who has been HIV-positive for 6 years. The antibodies have high affinity for antigen (mostly with affinity constants of > 10(8) M-1) and notable sequence diversity. Given appropriate donor selection, the methods described should allow the generation of antibodies for the evaluation of passive immunization as a therapy for AIDS.
引用
收藏
页码：10134 / 10137
页数：4
相关论文
共 24 条
[1]   ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE [J].
BARBAS, CF ;
KANG, AS ;
LERNER, RA ;
BENKOVIC, SJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (18) :7978-7982
[2]   PROTECTION OF CHIMPANZEES FROM INFECTION BY HIV-1 AFTER VACCINATION WITH RECOMBINANT GLYCOPROTEIN GP120 BUT NOT GP160 [J].
BERMAN, PW ;
GREGORY, TJ ;
RIDDLE, L ;
NAKAMURA, GR ;
CHAMPE, MA ;
PORTER, JP ;
WURM, FM ;
HERSHBERG, RD ;
COBB, EK ;
EICHBERG, JW .
NATURE, 1990, 345 (6276) :622-625
[3]   HUMAN AND MOUSE MONOCLONAL-ANTIBODIES BY REPERTOIRE CLONING [J].
BURTON, DR .
TRENDS IN BIOTECHNOLOGY, 1991, 9 (05) :169-175
[4]   INFLUENZA-VIRUS HEMAGGLUTININ-SPECIFIC ANTIBODIES ISOLATED FROM A COMBINATORIAL EXPRESSION LIBRARY ARE CLOSELY RELATED TO THE IMMUNE-RESPONSE OF THE DONOR [J].
CATON, AJ ;
KOPROWSKI, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6450-6454
[5]  
CATON AJ, 1991, P NATL ACAD SCI USA, V88, P1590
[6]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[7]   MAKING ANTIBODY FRAGMENTS USING PHAGE DISPLAY LIBRARIES [J].
CLACKSON, T ;
HOOGENBOOM, HR ;
GRIFFITHS, AD ;
WINTER, G .
NATURE, 1991, 352 (6336) :624-628
[8]   VERTICAL TRANSMISSION OF HUMAN-IMMUNODEFICIENCY-VIRUS IS CORRELATED WITH THE ABSENCE OF HIGH-AFFINITY AVIDITY MATERNAL ANTIBODIES TO THE GP120 PRINCIPAL NEUTRALIZING DOMAIN [J].
DEVASH, Y ;
CALVELLI, TA ;
WOOD, DG ;
REAGAN, KJ ;
RUBINSTEIN, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (09) :3445-3449
[9]   ANTIBODY-MEDIATED INVITRO NEUTRALIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ABOLISHES INFECTIVITY FOR CHIMPANZEES [J].
EMINI, EA ;
NARA, PL ;
SCHLEIF, WA ;
LEWIS, JA ;
DAVIDE, JP ;
LEE, DR ;
KESSLER, J ;
CONLEY, S ;
MATSUSHITA, S ;
PUTNEY, SD ;
GERETY, RJ ;
EICHBERG, JW .
JOURNAL OF VIROLOGY, 1990, 64 (08) :3674-3678
[10]  
GOEDERT JJ, 1991, LANCET, V337, P1351
123