Expression of a transactivation-deficient form of thyroid transcription factor I decreases the activity of co-transfected thyroglobulin and thyroperoxidase promoters

被引：9

作者：

ChristopheHobertus, C ^{[1
]}

vanRenterghem, P ^{[1
]}

Pichon, B ^{[1
]}

Christophe, D ^{[1
]}

机构：

[1] FREE UNIV BRUSSELS,FAC MED,IRIBHN,B-1070 BRUSSELS,BELGIUM

来源：

FEBS LETTERS | 1996年 / 399卷 / 1-2期

关键词：

TTF-1; homeodomain; thyroglobulin; thyroperoxidase; transcription; thyroid;

D O I：

10.1016/S0014-5793(96)01308-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Thyroid transcription factor I(TTF-1) plays a critical role in thyroid organogenesis and in the control of expression of several thyroid-specific genes, like those coding for thyroglobulin and thyroperoxidase. We have expressed the isolated DNA-binding homeodomain of TTF-I in cultured thyroid cells by transient transfection. A specific reduction in the activity of cotransfected thyroglobulin and thyroperoxidase promoters was observed in the presence of the isolated TTF-1 homeodomain, as compared to their activity measured in the presence of a mutated homeodomain unable to bind DNA. The activity of the SV40 early promoter, used as a control, was only marginally affected in these experiments. The transactivation-deficient form of TTF-1 described here may thus be used for investigating other cellular processes that are dependent on TTF-1 transcriptional activity.

引用

页码：140 / 142

页数：3

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