Heterologous production of bisucaberin using a biosynthetic gene cluster cloned from a deep sea metagenome

被引：26

作者：

Fujita, Masaki J. ^{[1
]}

Kimura, Nobutada ^{[2
]}

Yokose, Hisayoshi ^{[3
]}

Otsuka, Masami ^{[4
]}

机构：

[1] Kumamoto Univ, Prior Org Innovat & Excellence, Kumamoto 8620973, Japan

[2] Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki 3058568, Japan

[3] Kumamoto Univ, Fac Sci, Kumamoto 8620973, Japan

[4] Kumamoto Univ, Grad Sch Life Sci, Kumamoto 8620973, Japan

来源：

MOLECULAR BIOSYSTEMS | 2012年 / 8卷 / 02期

关键词：

CATALYZES CYCLODIMERIZATION; ENVIRONMENTAL DNA; SIDEROPHORE; IDENTIFICATION; PUTREBACTIN; DIVERSITY; ALCALIGIN; LIBRARIES; CLONING; CELLS;

D O I：

10.1039/c1mb05431g

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A siderophore biosynthetic gene cluster was cloned from a metagenomic library generated from deep sea sediment. The gene cluster was successfully expressed in Escherichia coli to produce bisucaberin, a siderophore originally reported from the marine bacterium Alteromonas haloplanktis. The cloned bisucaberin biosynthetic gene cluster was moderately similar to that of the known bisucaberin producer Vibrio salmonicida. However, the cloned gene cluster consists of four genes rather than three genes found in the V. salmonicida cluster. The low overall homology of the amino acid and nucleotide sequences with those of other species suggests that the cloned genes were derived from one of the unsequenced bacteria including uncultured species.

引用

页码：482 / 485

页数：4

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