PDK1 plays a critical role in regulating cardiac function in mice and human

被引：20

作者：

Di Ruo-min ^{[1
,2
]}

Feng Qiu-ting ^{[1
,2
]}

Chang Zai ^{[2
]}

Luan Qing ^{[2
]}

Zhang Yang-yang ^{[1
]}

Huang Jun ^{[1
]}

Li Xin-li ^{[1
]}

Yang Zhong-zhou ^{[2
]}

机构：

[1] Nanjing Med Univ, Dept Cardiol, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Univ, MOE Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing 210061, Jiangsu, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2010年 / 123卷 / 17期

基金：

美国国家科学基金会;

关键词：

PDK1; Akt; heart failure; FAILING HUMAN HEART; KINASE; AKT/GSK-3-BETA; SENSITIVITY; PATHWAYS; FAILURE; INSULIN;

D O I：

10.3760/cma.j.issn.0366-6999.2010.17.014

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background PDK1 is an essential protein kinase that plays a critical role in mammalian development. Mouse lacking PDK1 leads to multiple abnormalities and embryonic lethality at E9.5. To elucidate the role of PDK1 in the heart, we investigated the cardiac phenotype of mice that lack PDK1 in the heart in different growth periods and the alteration of PDK1 signaling in human failing heart. Methods We employed Cre/loxP system to generate PDK1(flox/flox): alpha-MHC-Cre mice, which specifically deleted PDK1 in cardiac muscle at birth, and tamoxifen-inducible heart-specific PDK1 knockout mice (PDK1(flox/flox):MerCreMer mice), in which PDK1 was deleted in myocardium in response to the treatment with tamoxifen. Transmural myocardial tissues from human failing hearts and normal hearts were sampled from the left ventricular apex to analyze the activity of PDK1/Akt signaling pathways by Western blotting. Results PDK1(flox/flox): alpha-MHC-Cre mice died of heart failure at 5 and 10 weeks old. PDK1(flox/flox)-MerCreMer mice died of heart failure from 5 to 21 weeks after the initiation of tamoxifen treatment at 8 weeks old. We found that expression levels of PDK1 in human failing heart tissues were significantly decreased compared with control hearts. Conclusion Our results suggest that PDK1 signaling network takes part in regulating cardiac viability and function in mice, and may be also involved in human heart failure disease. Chin Med J 2010;123(17):2358-2363

引用

页码：2358 / 2363

页数：6

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