Excitation wave propagation as a possible mechanism for signal transmission in pancreatic islets of Langerhans

被引:56
作者
Aslanidi, OV
Mornev, OA
Skyggebjerg, O
Arkhammar, P
Thastrup, O
Sorensen, MP
Christiansen, PL
Conradsen, K
Scott, AC
机构
[1] Tech Univ Denmark, Dept Math Modelling, DK-2800 Lyngby, Denmark
[2] RAS, Inst Cell Biophys, Pushchino 142290, Moscow Region, Russia
[3] RAS, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Region, Russia
[4] Novo Nordisk AS, Dept Cell Biol, DK-2820 Gentofte, Denmark
[5] Bioimage AS, DK-2860 Soborg, Denmark
关键词
D O I
10.1016/S0006-3495(01)76096-1
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
In response to glucose application, beta -cells forming pancreatic islets of Langerhans start bursting oscillations of the membrane potential and intracellular calcium concentration, inducing insulin secretion by the cells, Until recently, it has been assumed that the bursting activity of beta -cells in a single islet of Langerhans is synchronized across the whole islet due to coupling between the cells. However, time delays of several seconds in the activity of distant cells are usually observed in the islets of Langerhans, indicating that electrical/calcium wave propagation through the islets can occur. This work presents both experimental and theoretical evidence for wave propagation in the islets of Langerhans. Experiments with Fura-2 fluorescence monitoring of spatiotemporal calcium dynamics in the islets have clearly shown such wave propagation. Furthermore, numerical simulations of the model describing a cluster of electrically coupled beta -cells have supported our view that the experimentally observed calcium waves are due to electric pulses propagating through the cluster. This point of view is also supported by independent experimental results. Based on the model equations, an approximate analytical expression for the wave velocity is introduced, indicating which parameters can alter the velocity. We point to the possible role of the observed waves as signals controlling the insulin secretion inside the islets of Langerhans, in particular, in the regions that cannot be reached by any external stimuli such as high glucose concentration outside the islets.
引用
收藏
页码:1195 / 1209
页数:15
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