Bis-pyrene labeled DNA aptamer as an intelligent fluorescent biosensor

被引:73
作者
Yamana, K
Ohtani, Y
Nakano, H
Saito, I
机构
[1] Himeji Inst Technol, Dept Appl Chem, Himeji, Hyogo 6712201, Japan
[2] Kyoto Univ, Dept Synthet Chem & Biol Chem, Sakyo Ku, Kyoto 6068501, Japan
关键词
D O I
10.1016/S0960-894X(03)00799-6
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The site-directed incorporation of bis-pyrenyl fluorophore into anti-ATP DNA aptamer results in a creation of an intelligent fluorescent sensor with high signal intensity and specificity for detecting the target ligand in a homogeneous system. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3429 / 3431
页数:3
相关论文
共 12 条
[1]   Aptamer beacons for the direct detection of proteins [J].
Hamaguchi, N ;
Ellington, A ;
Stanton, M .
ANALYTICAL BIOCHEMISTRY, 2001, 294 (02) :126-131
[2]   Biochemistry - Adaptive recognition by nucleic acid aptamers [J].
Hermann, T ;
Patel, DJ .
SCIENCE, 2000, 287 (5454) :820-825
[3]   A DNA APTAMER THAT BINDS ADENOSINE AND ATP [J].
HUIZENGA, DE ;
SZOSTAK, JW .
BIOCHEMISTRY, 1995, 34 (02) :656-665
[4]   A review of molecular recognition technologies for detection of biological threat agents [J].
Iqbal, SS ;
Mayo, MW ;
Bruno, JG ;
Bronk, BV ;
Batt, CA ;
Chambers, JP .
BIOSENSORS & BIOELECTRONICS, 2000, 15 (11-12) :549-578
[5]   In vitro selection of signaling aptamers [J].
Jhaveri, S ;
Rajendran, M ;
Ellington, AD .
NATURE BIOTECHNOLOGY, 2000, 18 (12) :1293-1297
[6]   Designed signaling aptamers that transduce molecular recognition to changes in fluorescence intensity [J].
Jhaveri, SD ;
Kirby, R ;
Conrad, R ;
Maglott, EJ ;
Bowser, M ;
Kennedy, RT ;
Glick, G ;
Ellington, AD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (11) :2469-2473
[7]   Structural basis of DNA folding and recognition in an AMP-DNA aptamer complex: distinct architectures but common recognition motifs for DNA and RNA aptamers complexed to AMP [J].
Lin, CH ;
Patel, DJ .
CHEMISTRY & BIOLOGY, 1997, 4 (11) :817-832
[8]  
NUTIU R, IN PRESS J AM CHEM S
[9]   Protein analysis on a proteomic scale [J].
Phizicky, E ;
Bastiaens, PIH ;
Zhu, H ;
Snyder, M ;
Fields, S .
NATURE, 2003, 422 (6928) :208-215
[10]   Aptamer-based folding fluorescent sensor for cocaine [J].
Stojanovic, MN ;
de Prada, P ;
Landry, DW .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (21) :4928-4931