The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells

被引：43

作者：

Barrett, P ^{[1
]}

Morris, MA ^{[1
]}

Moar, KM ^{[1
]}

Mercer, JG ^{[1
]}

Davidson, JA ^{[1
]}

Findlay, PA ^{[1
]}

Adam, CL ^{[1
]}

Morgan, PJ ^{[1
]}

机构：

[1] Rowett Res Inst, Aberdeen Ctr Energy Regulat & Obes, Mol Neuroendocrinol Grp, Aberdeen AB21 9SB, Scotland

来源：

JOURNAL OF NEUROENDOCRINOLOGY | 2001年 / 13卷 / 04期

关键词：

hypothalamus; neuropeptide; obesity; appetite; pituitary;

D O I：

10.1046/j.1365-2826.2001.00634.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The cocaine-amphetamine regulated transcript (CART) encodes for a protein which has an important role in the regulation of appetite and body weight. To date, no details of the molecular events and signal transduction pathways which regulate this gene are available. We report the identification of CART gene expression in the GH3 pituitary cell line. We have used activators of the cAMP or protein kinase C (PKC) signal transduction pathways to show that, in GH3 cells, CART is transcriptionally up-regulated by activators of the cAMP signal transduction pathway. We also identify CART gene expression in ovine pars tuberalis (PT) tissue and primary cell cultures. In PT cells in contrast to GH3 cells, CART gene expression is upregulated by activators of the PKC signal transduction pathway. Cultured cells have provided a valuable resource for the detailed analysis of specific regulatory mechanisms underlying transcriptional or translational regulation of genes, signal transduction events and many other cellular processes. GH3 and PT cells may therefore provide a resource for the further detailed molecular analysis of the events regulating CART gene expression and processing.

引用

页码：347 / 352

页数：6

共 21 条

[11] Leptin signaling in the hypothalamus of normal rats in vivo [J].

McCowen, KC ;

Chow, JC ;

Smith, RJ .

ENDOCRINOLOGY, 1998, 139 (11) :4442-4447

[12] oPer1 is an early response gene under photoperiodic regulation in the ovine pars tuberalis [J].

Morgan, PJ ;

Ross, AW ;

Graham, ES ;

Adam, C ;

Messager, S ;

Barrett, P .

JOURNAL OF NEUROENDOCRINOLOGY, 1998, 10 (05) :319-323

[13] The ovine pars tuberalis secretes a factor(s) that regulates gene expression in both lactotropic and nonlactotropic pituitary cells [J].

Morgan, PJ ;

Webster, CA ;

Mercer, JG ;

Ross, AW ;

Hazlerigg, DG ;

MacLean, A ;

Barrett, P .

ENDOCRINOLOGY, 1996, 137 (09) :4018-4026

[14]

RAWLINGS SR, 1994, J BIOL CHEM, V269, P5680

[15] Pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP/vasoactive intestinal polypeptide receptors: Actions on the anterior pituitary gland [J].

Rawlings, SR ;

Hezareh, M .

ENDOCRINE REVIEWS, 1996, 17 (01) :4-29

[16] Pituitary adenylate cyclase-activating polypeptide transduces through cAMP/PKA and PKC pathways and stimulates proopiomelanocortin gene transcription in mouse melanotropes [J].

Rene, F ;

Monnier, D ;

Gaiddon, C ;

Felix, JM ;

Loeffler, JP .

NEUROENDOCRINOLOGY, 1996, 64 (01) :2-13

[17]

SEDIAH NG, 1992, NIDA RES MONOGR, V216, P132

[18] PITUITARY PROOPIOMELANOCORTIN GENE-EXPRESSION REQUIRES SYNERGISTIC INTERACTIONS OF SEVERAL REGULATORY ELEMENTS [J].

THERRIEN, M ;

DROUIN, J .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (07) :3492-3503

[19] CELL-SPECIFIC HELIX-LOOP-HELIX FACTOR REQUIRED FOR PITUITARY EXPRESSION OF THE PROOPIOMELANOCORTIN GENE [J].

THERRIEN, M ;

DROUIN, J .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (04) :2342-2353

[20] Leptin activation of Stat3 in the hypothalamus of wildtype and ob/ob mice but not db/db mice [J].

Vaisse, C ;

Halaas, JL ;

Horvath, CM ;

Darnell, JE ;

Stoffel, M ;

Friedman, JM .

NATURE GENETICS, 1996, 14 (01) :95-97

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