Activation of a protease cascade involved in patterning the Drosophila embryo

被引:74
作者
LeMosy, EK [1 ]
Tan, YQ [1 ]
Hashimoto, C [1 ]
机构
[1] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA
关键词
D O I
10.1073/pnas.081026598
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Dorsoventral patterning of the Drosophila embryo is initiated by a ventralizing signal. Production of this signal requires the serine proteases Gastrulation Defective (GD), Snake, and Easter, which genetic studies suggest act sequentially in a cascade that is activated locally in response to a ventral cue provided by the pipe gene. Here, we demonstrate biochemically that GD activates Snake, which in turn activates Easter. We also provide evidence that GD zymogen cleavage is important for triggering this cascade but is not spatially localized by pipe. Our results suggest that a broadly, rather than locally, activated protease cascade produces the ventralizing signal, so a distinct downstream step in this cascade must be spatially regulated to restrict signaling to the ventral side of the embryo.
引用
收藏
页码:5055 / 5060
页数:6
相关论文
共 24 条
[1]   Pinning down positional information:: Dorsal-ventral polarity in the Drosophila embryo [J].
Anderson, KV .
CELL, 1998, 95 (04) :439-442
[2]   CHARACTERIZATION AND USE OF THE DROSOPHILA METALLOTHIONEIN PROMOTER IN CULTURED DROSOPHILA-MELANOGASTER CELLS [J].
BUNCH, TA ;
GRINBLAT, Y ;
GOLDSTEIN, LSB .
NUCLEIC ACIDS RESEARCH, 1988, 16 (03) :1043-1061
[3]  
CHASAN R, 1992, DEVELOPMENT, V115, P607
[4]   Proteolytic processing of the Drosophila Spatzle protein by Easter generates a dimeric NGF-like molecule with ventralising activity [J].
DeLotto, Y ;
DeLotto, R .
MECHANISMS OF DEVELOPMENT, 1998, 72 (1-2) :141-148
[5]   Feedback control of intercellular signalling in development [J].
Freeman, M .
NATURE, 2000, 408 (6810) :313-319
[6]   THE MOLECULAR-BASIS OF BLOOD-COAGULATION [J].
FURIE, B ;
FURIE, BC .
CELL, 1988, 53 (04) :505-518
[7]   Gastrulation defective is a serine protease involved in activating the receptor Toll to polarize the Drosophila embryo [J].
Han, JF ;
Lee, SH ;
Tan, YQ ;
LeMosy, EK ;
Hashimoto, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (16) :9093-9097
[8]  
Harlow E., 1999, USING ANTIBODIES LAB, P352
[9]  
HASHIMOTO C, 1991, DEVELOPMENT, V111, P1021
[10]  
HECHT P M, 1992, Trends in Cell Biology, V2, P197, DOI 10.1016/0962-8924(92)90246-J