Cloning, mRNA localization and evolutionary conservation of a human 5-HT7 receptor pseudogene

被引：35

作者：

Olsen, MA ^{[1
]}

Schechter, LE ^{[1
]}

机构：

[1] Wyeth Ayerst Res, CNS Disorders, Princeton, NJ 08543 USA

来源：

GENE | 1999年 / 227卷 / 01期

关键词：

G protein-coupled receptor; retrotransposon; serotonin receptor; species homologue;

D O I：

10.1016/S0378-1119(98)00588-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Initial experiments designed to clone novel serotonin receptor subtypes in the substantia nigra have led to the discovery of a transcribed human 5-HT7 receptor pseudogene that is expressed in a wide range of tissues. The original clone (S771) possessed greater than 90% homology to the 5-HT7 receptor sequence and was identified by a degenerate PCR approach. Expression of the pseudogene transcript was detected throughout the brain and peripheral tissues in general agreement with 5-HT7 mRNA localization. interestingly, the transcript was detected in tissues not known to express the 5-HT7 receptor (i.e. liver and kidney). Analysis of genomic DNA explained the genesis of the human pseudogene via a processed parental transcript (retrotransposition) and led to the discovery of a species homologue in the rhesus monkey. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：63 / 69

页数：7

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