Short-term temporal variability in airborne bacterial and fungal populations

被引:202
作者
Fierer, Noah [1 ,2 ]
Liu, Zongzhi [3 ]
Rodriguez-Hernandez, Mari [4 ]
Knight, Rob [3 ]
Henn, Matthew [5 ,6 ]
Hernandez, Mark T. [4 ]
机构
[1] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA
[2] Univ Colorado, Cooperat Inst Res Environm Sci, Boulder, CO 80309 USA
[3] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA
[4] Univ Colorado, Dept Civil Environm & Architectural Engn, Boulder, CO 80309 USA
[5] MIT, Broad Inst, Cambridge, MA 02142 USA
[6] Harvard Univ, Cambridge, MA 02142 USA
关键词
D O I
10.1128/AEM.01467-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Airborne microorganisms have been studied for centuries, but the majority of this research has relied on cultivation-dependent surveys that may not capture all of the microbial diversity in the atmosphere. As a result, our understanding of airborne microbial ecology is limited despite the relevance of airborne microbes to human health, various ecosystem functions, and environmental quality. Cultivation-independent surveys of small-subunit rRNA genes were conducted in order to identify the types of airborne bacteria and fungi found at a single site (Boulder, CO) and the temporal variability in the microbial assemblages over an 8-day period. We found that the air samples were dominated by ascomycete fungi of the Hypocreales order and a diverse array of bacteria, including members of the proteobacterial and Cytophaga-Flavobacterium-Bacteroides groups that are commonly found in comparable culture-independent surveys of airborne bacteria. Bacterium/fungus ratios varied by 2 orders of magnitude over the sampling period, and we observed large shifts in the phylogenetic diversity of bacteria present in the air samples collected on different dates, shifts that were not likely to be related to local meteorological conditions. We observed more phylogenetic similarity between bacteria collected from geographically distant sites than between bacteria collected from the same site on different days. These results suggest that outdoor air may harbor similar types of bacteria regardless of location and that the short-term temporal variability in airborne bacterial assemblages can be very large.
引用
收藏
页码:200 / 207
页数:8
相关论文
共 55 条
[51]   Methods for integrated air sampling and DNA analysis for detection of airborne fungal spores [J].
Williams, RH ;
Ward, E ;
McCartney, HA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2453-2459
[52]   High-density microarray of small-subunit ribosomal DNA probes [J].
Wilson, KH ;
Wilson, WJ ;
Radosevich, JL ;
DeSantis, TZ ;
Viswanathan, VS ;
Kuczmarski, TA ;
Andersen, GL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (05) :2535-2541
[53]   Methods to determine the biological composition of particulate matter collected from outdoor air [J].
Womiloju, TO ;
Miller, JD ;
Mayer, PM ;
Brook, JR .
ATMOSPHERIC ENVIRONMENT, 2003, 37 (31) :4335-4344
[54]   Sejongia antarctica gen. nov., sp nov and Sejongia jeonii sp nov., isolated from the Antarctic [J].
Yi, H ;
Yoon, HI ;
Chun, J .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2005, 55 :409-416
[55]   FastGroupII: A web-based bioinformatics platform for analyses of large 16S rDNA libraries [J].
Yu, YN ;
Breitbart, M ;
McNairnie, P ;
Rohwer, F .
BMC BIOINFORMATICS, 2006, 7 (1)