Single-molecule reactions in liposomes

被引：42

作者：

Hsin, Tse-Ming

Yeung, Edward S. ^{[1
]}

机构：

[1] Iowa State Univ, USDOE, Ames Lab, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 42期

关键词：

enzyme catalysis; fluorescence; liposomes; protein structures; single-molecule studies;

D O I：

10.1002/anie.200702348

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Solitary confinement: Single-molecule reactions were initiated by trapping and fusing liposomes and monitored by fluorescence imaging. The pictures show: an optical image of the liposome containing the enzyme (A), a fluorescence image of one labeled enzyme molecule in the liposome (B), an optical image of the enzyme and substrate liposomes prior to electrofusion (C), an optical image of the fused liposome (D), and a fluorescence image of the fused liposome after incubation (E). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：8032 / 8035

页数：4

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