Repeat induced point mutation in two asexual fungi, Aspergillus niger and Penicillium chrysogenum

被引:42
作者
Braumann, Ilka [1 ]
van den Berg, Marco [2 ]
Kempken, Frank [1 ]
机构
[1] Univ Kiel, Abt Botan Schwerpunkt Genet & Mol Biol, Botan Inst Bot Garten, D-24098 Kiel, Germany
[2] DSM Antiinfect 624 0270, NL-2613 AX Delft, Netherlands
关键词
repeat induced point mutation; Aspergillus niger; Penicillium chrysogenum; transposons;
D O I
10.1007/s00294-008-0185-y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Repeat induced point mutation (RIP) is a gene silencing mechanism present in fungal genomes. During RIP, duplicated sequences are efficiently and irreversibly mutated by transitions from C:G to T:A. For the first time, we have identified traces of RIP in transposable elements of Aspergillus niger and Penicillium chrysogenum, two biotechnologically relevant fungi. We found that RIP in P. chrysogenum has affected a large set of sequences, which also contain other mutations. On the other hand, RIP in A. niger is limited to only few sequences, but literally all mutations are RIP-like. Surprisingly, RIP occurred only in transposon sequences that have disrupted open reading frames in A. niger, a phenomenon not yet reported for other fungi. In both fungal species, we identified two sequences with strong sequence similarity to Neurospora crassa RID. RID is a putative DNA methyltransferase and the only known enzyme involved in the RIP process. Our findings suggest that both A. niger and P. chrysogenum either had a sexual past or have a sexual potential. These findings have important implications for future strain development of these fungi.
引用
收藏
页码:287 / 297
页数:11
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