The relative effect of citral on mitotic microtubules in wheat roots and BY2 cells

被引:40
作者
Chaimovitsh, D. [2 ,3 ]
Rogovoy , O. [1 ,3 ]
Altshuler, O. [1 ]
Belausov, E. [1 ]
Abu-Abied, M. [1 ]
Rubin, B. [3 ]
Sadot, E. [1 ]
Dudai, N. [2 ]
机构
[1] Agr Res Org, Volcani Ctr, Inst Plant Sci, IL-50250 Bet Dagan, Israel
[2] ARO, Div Aromat Plants, Newe Yaar, Ramat Yishai, Israel
[3] Hebrew Univ Jerusalem, RH Smith Inst Plant Sci & Genet Agr, Fac Agr Food & Environm, IL-76100 Rehovot, Israel
关键词
Cell division; monoterpene; phragmoplast; preprophase band; spindle; wheat; GAMMA-TUBULIN COMPLEX; DIVISION PLANE; DINITROANILINE HERBICIDE; PREPROPHASE BANDS; ESSENTIAL OILS; ARABIDOPSIS; SPINDLE; ORGANIZATION; NUCLEATION; GROWTH;
D O I
10.1111/j.1438-8677.2011.00511.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plant volatile monoterpene citral is a highly active compound with suggested allelopathic traits. Seed germination and seedling development are inhibited in the presence of citral, and it disrupts microtubules in both plant and animal cells in interphase. We addressed the following additional questions: can citral interfere with cell division; what is the relative effect of citral on mitotic microtubules compared to interphase cortical microtubules; what is its effect on newly formed cell plates; and how does it affect the association of microtubules with ?-tubulin? In wheat seedlings, citral led to inhibition of root elongation, curvature of newly formed cell walls and deformation of microtubule arrays. Citrals effect on microtubules was both dose- and time-dependent, with mitotic microtubules appearing to be more sensitive to citral than cortical microtubules. Association of ?-tubulin with microtubules was more sensitive to citral than were the microtubules themselves. To reveal the role of disrupted mitotic microtubules in dictating aberrations in cell plates in the presence of citral, we used tobacco BY2 cells expressing GFP-Tua6. Citral disrupted mitotic microtubules, inhibited the cell cycle and increased the frequency of asymmetric cell plates in these cells. The time scale of citrals effect in BY2 cells suggested a direct influence on cell plates during their formation. Taken together, we suggest that at lower concentrations, citral interferes with cell division by disrupting mitotic microtubules and cell plates, and at higher concentrations it inhibits cell elongation by disrupting cortical microtubules.
引用
收藏
页码:354 / 364
页数:11
相关论文
共 63 条
[1]   Mitotic Spindle Organization by the Preprophase Band [J].
Ambrose, J. Christian ;
Cyr, Richard .
MOLECULAR PLANT, 2008, 1 (06) :950-960
[2]   MECHANISMS OF CITRAL PHOTOTOXICITY [J].
ASTHANA, A ;
LARSON, RA ;
MARLEY, KA ;
TUVESON, RW .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1992, 56 (02) :211-222
[3]   γ-Tubulin is essential for acentrosomal microtubule nucleation and coordination of late mitotic events in Arabidopsis [J].
Binarová, P ;
Cenklová, V ;
Procházková, J ;
Doskocilová, A ;
Volc, J ;
Vrlík, M ;
Bögre, L .
PLANT CELL, 2006, 18 (05) :1199-1212
[4]   Microtubules are an intracellular target of the plant terpene citral [J].
Chaimovitsh, David ;
Abu-Abied, Mohamad ;
Belausov, Eduard ;
Rubin, Baruch ;
Dudai, Nativ ;
Sadot, Einat .
PLANT JOURNAL, 2010, 61 (03) :399-408
[5]   Localization of the microtubule end binding protein EB1 reveals alternative pathways of spindle development in Arabidopsis suspension cells [J].
Chan, J ;
Calder, G ;
Fox, S ;
Lloyd, C .
PLANT CELL, 2005, 17 (06) :1737-1748
[6]   Citral inhibits cell proliferation and induces apoptosis and cell cycle arrest in MCF-7 cells [J].
Chaouki, Wahid ;
Leger, David Y. ;
Liagre, Bertrand ;
Beneytout, Jean-Louis ;
Hmamouchi, Mohamed .
FUNDAMENTAL & CLINICAL PHARMACOLOGY, 2009, 23 (05) :549-556
[7]   Structural basis for the interaction of tubulin with proteins and drugs that affect microtubule dynamics [J].
Downing, KH .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2000, 16 :89-111
[8]   Essential oils as allelochemicals and their potential use as bioherbicides [J].
Dudai, N ;
Poljakoff-Mayber, A ;
Mayer, AM ;
Putievsky, E ;
Lerner, HR .
JOURNAL OF CHEMICAL ECOLOGY, 1999, 25 (05) :1079-1089
[9]   Citral is a new inducer of caspase-3 in tumor cell lines [J].
Dudai, N ;
Weinstein, Y ;
Krup, M ;
Rabinski, T ;
Ofir, R .
PLANTA MEDICA, 2005, 71 (05) :484-488
[10]   Biotransformation of constituents of essential oils by germinating wheat seed [J].
Dudai, N ;
Larkov, O ;
Putievsky, E ;
Lerner, HR ;
Ravid, U ;
Lewinsohn, E ;
Mayer, AR .
PHYTOCHEMISTRY, 2000, 55 (05) :375-382