ALASKA:: A Mathematica package for two-state kinetic analysis of protein folding reactions

被引：14

作者：

Burton, RE ^{[1
]}

Busby, RS ^{[1
]}

Oas, TG ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA

来源：

JOURNAL OF BIOMOLECULAR NMR | 1998年 / 11卷 / 03期

关键词：

dynamic NMR; kinetics; line shape simulation; protein folding;

D O I：

10.1023/A:1008276703114

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A Mathematica package (ALASKA) has been developed to simplify the measurement of protein folding kinetics by analysis of H-1 NMR lineshape analysis. This package reads NMR data in ASCII format and can simulate an aromatic (NMR)-N-1 spectrum with or without lineshape broadening from chemical exchange. We describe the analysis of a urea denaturation series of a fast-folding protein, the G46A/G48A variant of monomeric lambda repressor.

引用

页码：355 / 359

页数：5

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