Mechanism of the interaction between bromophenol blue and bovine serum albumin

被引：18

作者：

Cao, WG

Jiao, QC ^{[1
]}

Fu, Y

Chen, L

Liu, Q

机构：

[1] Nanjing Univ, Coll Life Sci, Pharmaceut Biotechnol Key Lab, Nanjing 210093, Peoples R China

[2] Suzhou Teachers Coll, Dept Biochem, Suzhou, Anhui, Peoples R China

来源：

SPECTROSCOPY LETTERS | 2003年 / 36卷 / 03期

关键词：

bromophenol blue; bovine serum albumin; spectrophotometry; interaction mechanism;

D O I：

10.1081/SL-120024351

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

The mechanism of the interaction between Bromophenol Blue (BPB) and Bovine Serum Albumin (BSA) was studied spectrophotometric ally. Absorption spectral changes of BPB-BSA and BPB-Chitosan mixtures in different BSA and Chitosan concentrations are discussed. The influence of different experimental conditions on the absorption spectra of BPB-BSA mixtures was investigated. Through these experiments, the conclusion was drawn that the bathochromic shift of the absorption spectra of the BPB-BSA reaction system results mainly from hydrophobic interaction between BPB and BSA, while electrostatic force is necessary for the formation of the BPB-BSA complex.

引用

页码：197 / 209

页数：13

共 23 条

[1] INTERACTION OF BROMPHENOL BLUE AND BILIRUBIN WITH BOVINE AND HUMAN SERUM ALBUMIN DETERMINED BY GEL FILTRATION [J].