Phylogeny and characterization of three nifH-homologous genes from Paenibacillus azotofixans

被引：28

作者：

Choo, QC ^{[1
]}

Samian, MR ^{[1
]}

Najimudin, N ^{[1
]}

机构：

[1] Univ Sains Malaysia, Sch Biol Sci, George Town 11800, Malaysia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 06期

关键词：

D O I：

10.1128/AEM.69.6.3658-3662.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper, we report the cloning and characterization of three Paenibacillus azotofixans DNA regions containing genes involved in nitrogen fixation. Sequencing analysis revealed the presence of nijB1H1D1K1 gene organization in the 4,607-bp SacI DNA fragment. This is the first report of linkage of a nifB open reading frame upstream of the structural nif genes. The second (nifB2H2) and third (nifH3) nif homologues are confined within the 6,350-bp HindIII and 2,840-bp EcoRI DNA fragments, respectively. Phylogenetic analysis demonstrated that NifH1 and NifH2 form a monophyletic group among cyanobacterial NifH proteins. NifH3, on the other hand, clusters among NifH proteins of the highly divergent methanogenic archaea.

引用

页码：3658 / 3662

页数：5

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