Real-time quaking-induced conversion A highly sensitive assay for prion detection

被引：125

作者：

Atarashi, Ryuichiro ^{[1
]}

Sano, Kazunori ^{[1
,2
]}

Satoh, Katsuya ^{[1
]}

Nishida, Noriyuki ^{[1
,2
]}

机构：

[1] Nagasaki Univ, Grad Sch Biomed Sci, Dept Mol Microbiol & Immunol, Nagasaki 852, Japan

[2] Nagasaki Univ, Global COE Program, Nagasaki 852, Japan

来源：

PRION | 2011年 / 5卷 / 03期

关键词：

RT-QUIC; real-time quaking-induced conversion; prion; CJD; Creutzfeldt-Jakob disease; CSF; cerebrospinal fluid; CREUTZFELDT-JAKOB-DISEASE; CEREBROSPINAL-FLUID; SEEDED CONVERSION; AMYLOID FIBRILS; 14-3-3; PROTEIN; AMPLIFICATION; DIAGNOSIS;

D O I：

10.4161/pri.5.3.16893

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently developed a new in vitro amplification technology, designated "real-time quaking-induced conversion (RT-QUIC)," for detection of the abnormal form of prion protein (PrPSc) in easily accessible specimens such as cerebrospinal fluid (CSF). After assessment of more than 200 CSF specimens from Japanese and Australian patients, we found no instance of a false positive and more than 80% accuracy for the correct diagnosis of sporadic Creutzfeldt-Jakob disease (sCJD). Furthermore, the RT-QUIC can be applied to other prion diseases, including scrapie, chronic wasting disease (CWD) and bovine spongiform encephalopathy (BSE), and is able to quantify prion seeding activity when combined with an end-point dilution of samples. These results indicate that the RT-QUIC, with its high sensitivity and specificity, will be of great use as an early, rapid and specific assay for prion diseases.

引用

页码：150 / 153

页数：4

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