Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on Pseudomonas aeruginosa

被引:61
作者
Small, David A.
Chang, Wook
Toghrol, Freshteh [1 ]
Bentley, William E.
机构
[1] US EPA, Biol & Econ Anal Div, Microelect Res Lab, Off Pesticide Programs, Ft George G Meade, MD 20755 USA
[2] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore, Singapore
[3] Univ Maryland, Ctr Biosyst Res, Inst Biotechnol, College Pk, MD 20742 USA
关键词
Pseudomonas aeruginosa; sodium hypochlorite; peracetic acid; hydrogen peroxide; microarrays;
D O I
10.1007/s00253-007-1072-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Disinfectants are routinely used in hospitals and health care facilities for surface sterilization. However, the mechanisms by which these disinfectants kill and the extent to which bacteria, including Pseudomonas aeruginosa, are resistant remains unclear. Consequently, P. aeruginosa nosocomial infections result in considerable casualties and economic hardship. Previously, DNA microarrays were utilized to analyze the genome-wide transcription changes in P. aeruginosa after oxidative antimicrobial (sodium hypochlorite, peracetic acid, and hydrogen peroxide) exposure. Simultaneous analysis of these transcriptome datasets provided a comprehensive understanding of the differential responses to these disinfectants. An analysis of variance, functional classification analysis, metabolic pathway analysis, Venn diagram analysis, and principal component analysis revealed that sodium hypochlorite exposure resulted in more genome-wide changes than either peracetic acid or hydrogen peroxide exposures.
引用
收藏
页码:1093 / 1105
页数:13
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