Detection of Borrelia burgdorferi by DNA amplification in synovial tissue samples from patients with lyme arthritis

被引:89
作者
Jaulhac, B
CharyValckenaere, I
Sibilia, J
Javier, RM
Piemont, Y
Kuntz, JL
Monteil, H
Pourel, J
机构
[1] CHU NANCY,NANCY,FRANCE
[2] HOP UNIV STRASBOURG,STRASBOURG,FRANCE
来源
ARTHRITIS AND RHEUMATISM | 1996年 / 39卷 / 05期
关键词
D O I
10.1002/art.1780390505
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. To compare the detection rates of chromosomal flagellin gene from Borrelia burgdorferi in synovial tissue (ST) and synovial fluid (SF) using polymerase chain reaction (PCR) techniques. Methods. B burgdorferi DNA was sought in SF and ST from 12 consecutive patients with Lyme arthritis and from 29 patients with noninfectious diseases (controls). Results. No DNA amplification was observed in samples obtained from the 29 control patients, whereas B burgdorferi DNA was detected in all ST and/or SF samples from the 12 patients with Lyme arthritis. Results from 1 ST sample were not interpretable because of PCR inhibitors. Among the 11 remaining patients, 10 had positive ST samples, whereas only 4 had positive SF samples (P < 0.05). Conclusion. These data suggest that detection of chromosomal B burgdorferi DNA may be more efficient in ST than SF.
引用
收藏
页码:736 / 745
页数:10
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