A tri-hybrid system for the analysis and detection of RNA-protein interactions

被引：67

作者：

Putz, U

Skehel, P

Kuhl, D

机构：

[1] UNIV HAMBURG,CTR MOL NEUROBIOL ZMNH,D-20246 HAMBURG,GERMANY

[2] NATL INST MED RES,DIV NEUROPHYSIOL,LONDON NW7 1AA,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 23期

关键词：

D O I：

10.1093/nar/24.23.4838

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A modification of the two-hybrid system is described for the in vivo reconstruction of specific RNA-protein interactions, In this tri-hybrid system, the DNA binding and transcription activation domains of the yeast transcriptional activator GAL4 are brought together via the interaction of recombinant fusion proteins with a recombinant RNA, The method provides a system for studying RNA-protein interactions with the genetic advantages of the two-hybrid system, It may be used to detect specific RNA-binding proteins or target RNAs from a library of cDNAs, or to analyse the structural specificity of identified RNA-protein interactions.

引用

页码：4838 / 4840

页数：3

共 18 条

[11] ARC, A GROWTH-FACTOR AND ACTIVITY-REGULATED GENE, ENCODES A NOVEL CYTOSKELETON-ASSOCIATED PROTEIN THAT IS ENRICHED IN NEURONAL DENDRITES [J].