A genetic basis for corneal sensitivity to ultraviolet light among recombinant SWXJ inbred strains of mice

被引:12
作者
Downes, JE
Swann, PG
Holmes, RS
机构
[1] GRIFFITH UNIV,DIV SCI & TECHNOL,BRISBANE,QLD 4111,AUSTRALIA
[2] QUEENSLAND UNIV TECHNOL,SCH OPTOMETRY,BRISBANE,QLD 4059,AUSTRALIA
关键词
alcohol dehydrogenase; aldehyde dehydrogenase; cornea; UV light; recombinant inbred mouse;
D O I
10.1076/ceyr.16.6.539.5075
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose. To examine a possible genetic basis for corneal sensitivity to UV-B light exposure. Methods. To this end, adult male mice from the 14 SWXJ recombinant inbred albino strains (originating from SJL/J and SWR/J parental strains) were subjected to ultraviolet (UV) radiation exposure of 0.078 J/cm(2) and photographed four days post-exposure, to assess corneal opacity and the possible correlation with corneal aldehyde dehydrogenase (ALDH) activity, alcohol dehydrogenase (ADH) activity and soluble protein content. Results. Those recombinant strains that exhibited the SWR/J strain phenotype of having low levels of ALDH and decreased soluble protein levels also exhibited greater levels of corneal clouding after UV-exposure than the other strains, which exhibited ''normal'' levels of both ALDH activity and soluble protein in the cornea. Conclusions. These data support an hypothesis for a major role for ALDH in assisting the cornea to protect the eye against W-induced tissue damage.
引用
收藏
页码:539 / 546
页数:8
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