Human fortilin is a molecular target of dihydroartemisinin

被引：52

作者：

Fujita, Takayuki ^{[1
]}

Felix, Kumar ^{[1
]}

Pinkaew, Decha ^{[1
,2
]}

Hutadilok-Towatana, Nongporn ^{[2
]}

Liu, Zhihe ^{[1
]}

Fujise, Ken ^{[1
]}

机构：

[1] Univ Texas Galveston, Med Branch, Dept Internal Med, Div Cardiol, Galveston, TX 77555 USA

[2] Prince Songkla Univ, Fac Sci, Dept Biochem, Hat Yai 90112, Songkhla, Thailand

来源：

FEBS LETTERS | 2008年 / 582卷 / 07期

关键词：

fortilin; dihydroartemisinin; DHA;

D O I：

10.1016/j.febslet.2008.02.055

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dehydroartemisinin (DHA) is an effective anti-malaria agent. Fortilin is an anti-apoptotic molecule overexpressed in many human cancers. Here, we show that DHA binds human fortilin, increases the ubiquitination of fortilin, shortens fortilin's half-life in a proteasome-dependent fashion, and reduces cellular levels of fortilin in varieties of cells. DHA induced DNA fragmentation in U2OS cells in a fortilin-dependent manner. The fortilin-knocked-down cells were less susceptible-and fortilin-overexpressing cells more susceptible-to DHA than were wild-type cells, suggesting that apoptotic effects of DHA are-at least partly-conferred through fortilin. Together, these data suggest that fortilin is a molecular target of DHA. DHA and its derivative may prove to be viable anti-cancer agents in fortilin-overexpressing cancers. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1055 / 1060

页数：6

共 19 条

[1]

ANSTEY NM, 1993, LANCET, V341, P1035

[2] REACTION OF ANTIMALARIAL ENDOPEROXIDES WITH SPECIFIC PARASITE PROTEINS [J].

ASAWAMAHASAKDA, W ;

ITTARAT, I ;

PU, YM ;

ZIFFER, H ;

MESHNICK, SR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (08) :1854-1858

[3] Molecular cloning and expression of a mammalian homologue of a translationally controlled tumor protein (TCTP) gene from Penaeus monodon shrimp [J].

Bangrak, P ;

Graidist, P ;

Chotigeat, W ;

Phongdara, A .

JOURNAL OF BIOTECHNOLOGY, 2004, 108 (03) :219-226

[4] The Plasmodium falciparum translationally controlled tumor protein homolog and its reaction with the antimalarial drug artemisinin [J].

Bhisutthibhan, J ;

Pan, XQ ;

Hossler, PA ;

Walker, DJ ;

Yowell, CA ;

Carlton, J ;

Dame, JB ;

Meshnick, SR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (26) :16192-16198

[5] Immunoprecipitation of [3H]dihydroartemisinin translationally controlled tumor protein (TCTP) adducts from Plasmodium falciparum-infected erythrocytes by using anti-TCTP antibodies [J].

Bhisutthibhan, J ;

Meshnick, SR .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (08) :2397-2399

[6]

China Cooperative Research Group on Qinghaosu and its Derivatives as Antimalarials, 1982, J TRADIT CHIN MED, V2, P3

[7] Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels [J].

Frostell-Karlsson, Å ;

Remaeus, A ;

Roos, H ;

Andersson, K ;

Borg, P ;

Hämäläinen, M ;

Karlsson, R .

JOURNAL OF MEDICINAL CHEMISTRY, 2000, 43 (10) :1986-1992

[8] Communication - Preferential interaction of sentrin with a ubiquitin-conjugating enzyme, Ubc9 [J].

Gong, LM ;

Kamitani, T ;

Fujise, K ;

Caskey, LS ;

Yeh, ETH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (45) :28198-28201

[9] Antiapoptotic protein partners fortilin and MCL1 independently protect cells from 5-fluorouracil-induced cytotoxicity [J].

Graidist, P ;

Phongdara, A ;

Fujise, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (39) :40868-40875

[10] Dihydroartemisinin enhances radiosensitivity of human glioma cells in vitro [J].

Kim, SJ ;

Kim, MS ;

Lee, JW ;

Lee, CH ;

Yoo, H ;

Shin, SH ;

Park, MJ ;

Lee, SH .

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 2006, 132 (02) :129-135

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