Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression: role of miR-155

被引：207

作者：

Boesch-Saadatmandi, Christine ^{[1
]}

Loboda, Agnieszka ^{[2
]}

Wagner, Anika E. ^{[1
]}

Stachurska, Anna ^{[2
]}

Jozkowicz, Alicja ^{[2
]}

Dulak, Jozef ^{[2
]}

Doering, Frank ^{[1
]}

Wolffram, Siegfried ^{[3
]}

Rimbach, Gerald ^{[1
]}

机构：

[1] Univ Kiel, Inst Human Nutr & Food Sci, D-24098 Kiel, Germany

[2] Jagiellonian Univ, Dept Med Biotechnol, Fac Biochem Biophys & Biotechnol, PL-30387 Krakow, Poland

[3] Univ Kiel, Inst Anim Nutr & Physiol, D-24098 Kiel, Germany

来源：

JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 2011年 / 22卷 / 03期

基金：

英国惠康基金;

关键词：

Quercetin; Macrophage; Inflammation; Flavonoid metabolism; MicroRNA; ADHESION MOLECULE EXPRESSION; KAPPA-B ACTIVITY; FLAVONOID QUERCETIN; PLATELET-AGGREGATION; DIETARY FLAVONOIDS; ENDOTHELIAL-CELLS; APOE GENOTYPE; IN-VITRO; PLASMA; PATHWAY;

D O I：

10.1016/j.jnutbio.2010.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study the effect of quercetin and its major metabolites quercetin-3-glucuronide (Q3G) and isorhamnetin on inflammatory gene expression was determined in murine RAW264.7 macrophages stimulated with lipopolysaccharide. Quercetin and isorhamnetin but not Q3G significantly decreased mRNA and protein levels of tumor necrosis factor alpha. Furthermore a significant decrease in mRNA levels of interleukin 13, interleukin 6, macrophage inflammatory protein 1 alpha and inducible nitric oxide synthase was evident in response to the quercetin treatment. However Q3G did not affect inflammatory gene expression. Anti-inflammatory properties of quercetin and isorhamnetin were accompanied by an increase in heme oxygenase 1 protein levels, a downstream target of the transcription factor Nrf2, known to antagonize chronic inflammation. Furthermore, proinflammatory microRNA-155 was down-regulated by quercetin and isorhamnetin but not by Q3G. Finally, anti-inflammatory properties of quercetin were confirmed in vivo in mice fed quercetin-enriched diets (0.1 mg quercetin/g diet) over 6 weeks. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：293 / 299

页数：7

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