β-CATENIN ACCUMULATION AND S33F MUTATION OF CTNNB1 GENE IN COLORECTAL CANCER IN SAUDI ARABIA

Several risk factors associated with colorectal cancer (CRC) have been identified including beta-catenin/CTNNB1 hotspot mutations. The levels of beta-catenin within a cell are regulated via phosphorylation of the N terminus of beta-catenin by GSK-3 beta. Thus far three serines (S33, 37, 45) and one threonine (T41) are considered to be the substrates for GSK-3 beta phosphorylation. In the present investigation an attempt was made to study the role of beta-catenin mutations in exon-3 in 60 colorectal cancer patients from Kingdom of Saudi Arabia (KSA). The hot spot mutation region of beta-catenin exon 3 was evaluated in matched tumor and normal tissues using PCR and direct sequencing. Sequencing of exon 3 of the CTNNB1 gene revealed an activating mutation (S33F) in one of the tumor samples as compared to the normal tissue from the same patient where there was no such mutation found. Immunohistochemical staining showed the accumulation of beta-catenin protein both in cytoplasm and in the nuclei of cancer cells as compared to normal tissue.