β-CATENIN ACCUMULATION AND S33F MUTATION OF CTNNB1 GENE IN COLORECTAL CANCER IN SAUDI ARABIA

被引:16
作者
Alomar, Suliman Y. [1 ]
Mansour, Lamjed [1 ]
Abuderman, Abdulwahab [2 ]
Alkhuriji, Afrah [1 ]
Arafah, Maha [3 ]
Alwasel, Saleh [1 ]
Harrath, Abdel Halim [1 ]
Almutairi, Mikhlid [1 ]
Trayhyrn, Paul [1 ,4 ]
Dar, Javid Ahmad [5 ]
机构
[1] King Saud Univ, Dept Zool, Riyadh 11451, Saudi Arabia
[2] Prince Sattam bin Abdulaziz Univ, Coll Med, Basic Med Sci Dept, Al Kharj, Saudi Arabia
[3] King Saud Univ, Dept Pathol, Riyadh 11451, Saudi Arabia
[4] Univ Buckingham, Buckingham Inst Translat Med, Clore Lab, Buckingham, England
[5] King Saud Univ, Coll Sci, Cent Lab, Riyadh 11451, Saudi Arabia
关键词
colorectal cancer; beta-catenin; somatic mutation; SNP; PCR-sequencing; ADENOMATOUS POLYPOSIS-COLI; DESMOID TUMORS; APC MUTATIONS; UNITED-STATES; EXPRESSION; PHOSPHORYLATION; PROTEIN; EPIDEMIOLOGY; INACTIVATION; SURVIVAL;
D O I
10.5114/PJP.2016.61452
中图分类号
R36 [病理学];
学科分类号
100103 [病原生物学];
摘要
Several risk factors associated with colorectal cancer (CRC) have been identified including beta-catenin/CTNNB1 hotspot mutations. The levels of beta-catenin within a cell are regulated via phosphorylation of the N terminus of beta-catenin by GSK-3 beta. Thus far three serines (S33, 37, 45) and one threonine (T41) are considered to be the substrates for GSK-3 beta phosphorylation. In the present investigation an attempt was made to study the role of beta-catenin mutations in exon-3 in 60 colorectal cancer patients from Kingdom of Saudi Arabia (KSA). The hot spot mutation region of beta-catenin exon 3 was evaluated in matched tumor and normal tissues using PCR and direct sequencing. Sequencing of exon 3 of the CTNNB1 gene revealed an activating mutation (S33F) in one of the tumor samples as compared to the normal tissue from the same patient where there was no such mutation found. Immunohistochemical staining showed the accumulation of beta-catenin protein both in cytoplasm and in the nuclei of cancer cells as compared to normal tissue.
引用
收藏
页码:156 / 162
页数:7
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