Erythrocyte spectrin is an E2 ubiquitin conjugating enzyme

被引:25
作者
Kakhniashvili, DG
Chaudhary, T
Zimmer, WE
Bencsath, FA
Jardine, I
Goodman, SR [1 ]
机构
[1] Univ S Alabama, Coll Med, Dept Neurosci & Cell Biol, Mobile, AL 36688 USA
[2] Univ S Alabama, Coll Med, Dept Biochem & Mol Biol, Mobile, AL 36688 USA
[3] Univ S Alabama, Coll Med, Ctr Comprehens Sickle Cell, Mobile, AL 36688 USA
[4] Thermoquest, San Jose, CA 95134 USA
[5] US FDA, Gulf Coast Seafood Lab, Dauphin Isl, AL 36528 USA
关键词
D O I
10.1021/bi010176t
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The involvement of red blood cell spectrin in the ubiquitination process was studied. Spectrin was found to form two ubiquitin-associated derivatives, a DTT-sensitive ubiquitin adduct and a DTT-insensitive conjugate, characteristic intermediate and final products of the ubiquitination reaction cascade. In addition to spectrin and ubiquitin, ubiquitin-activating enzyme (El) and ATP were necessary and sufficient to form both the spectrin-ubiquitin adduct and conjugate. No exogenous ubiquitin-conjugating (E2) or ligase (E3) activities were required, suggesting that erythrocyte spectrin is an E2 ubiquitin-conjugating enzyme able to target itself. Both ubiquitin adduct and conjugate were linked to the alpha subunit of spectrin, suggesting that the ubiquitin-conjugating (UBC) domain and its target regions reside on the same subunit.
引用
收藏
页码:11630 / 11642
页数:13
相关论文
共 100 条
[11]   SIGNAL-INDUCED SITE-SPECIFIC PHOSPHORYLATION TARGETS I-KAPPA-B-ALPHA TO THE UBIQUITIN-PROTEASOME PATHWAY [J].
CHEN, ZJ ;
HAGLER, J ;
PALOMBELLA, VJ ;
MELANDRI, F ;
SCHERER, D ;
BALLARD, D ;
MANIATIS, T .
GENES & DEVELOPMENT, 1995, 9 (13) :1586-1597
[12]   SPECTRIN-ACTIN ASSOCIATIONS STUDIED BY ELECTRON-MICROSCOPY OF SHADOWED PREPARATIONS [J].
COHEN, CM ;
TYLER, JM ;
BRANTON, D .
CELL, 1980, 21 (03) :875-883
[13]  
COOK WJ, 1992, J BIOL CHEM, V267, P15116
[14]   UBIQUITIN IS CONJUGATED TO THE CYTOSKELETAL PROTEIN ALPHA-SPECTRIN IN MATURE ERYTHROCYTES [J].
CORSI, D ;
GALLUZZI, L ;
CRINELLI, R ;
MAGNANI, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) :8928-8935
[15]   Alteration of α-spectrin ubiquitination due to age-dependent changes in the erythrocyte membrane [J].
Corsi, D ;
Paiardini, M ;
Crinelli, R ;
Bucchini, A ;
Magnani, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 261 (03) :775-783
[16]  
CORSI D, 1997, J BIOL CHEM, V260, P3128
[17]   AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].
ENG, JK ;
MCCORMACK, AL ;
YATES, JR .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989
[18]  
FAGAN JM, 1986, J BIOL CHEM, V261, P5705
[19]   Mdm2 is a RING finger-dependent ubiquitin protein ligase for itself and p53 [J].
Fang, SY ;
Jensen, JP ;
Ludwig, RL ;
Vousden, KH ;
Weissman, AM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (12) :8945-8951
[20]   SPECTRIN PLUS BAND 4.1 CROSS-LINK ACTIN - REGULATION BY MICROMOLAR CALCIUM [J].
FOWLER, V ;
TAYLOR, DL .
JOURNAL OF CELL BIOLOGY, 1980, 85 (02) :361-376