Direct electron transfer and electrocatalysis of hemoglobin immobilized on graphene-Pt nanocomposite

被引：57

作者：

Feng, Xiaomiao ^{[1
]}

Li, Ruimei ^{[1
]}

Hu, Chenhui ^{[2
,3
]}

Hou, Wenhua ^{[2
,3
]}

机构：

[1] Nanjing Univ Posts & Telecommun, Sch Mat Sci & Engn, Inst Adv Mat, Jiangsu Key Lab Organ Elect & Informat Displays, Nanjing 210046, Peoples R China

[2] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Mesoscop Chem, Nanjing 210093, Peoples R China

[3] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2011年 / 657卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

Graphene; Pt; Hemoglobin; Direct electron transfer; Biosensor; DIRECT ELECTROCHEMISTRY; CARBON NANOTUBES; AQUEOUS DISPERSIONS; GRAPHITE OXIDE; CYTOCHROME-C; FILMS; SHEETS; NANOPARTICLES; REDUCTION; NANOSHEETS;

D O I：

10.1016/j.jelechem.2011.03.004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel hemoglobin (Hb) electrode has been presented through adsorbing lib onto graphene-Pt nanocomposite. The graphene-Pt with uniform sheet-like structure was obtained by microwave-assisted method. Positions of Soret absorption band showed lib retained its natural configuration after adsorption onto graphene-Pt. The electrochemical behavior of the immobilized Hb was studied by cyclic voltammetry. The immobilized Hb showed a pair of stable and well-defined peaks at the formal potential of -0.26 V. As a model, a kind of novel third generation biosensor for H(2)O(2) was developed. The resultant heme-protein electrode exhibited electrocatalytical responses to the reduction of H(2)O(2), excellent stability, interference and good reproducibility. The presence of graphene enhanced the protein loadings, accelerates interfacial electron transfer and improves thermal stability of the adsorbed protein. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：28 / 33

页数：6

共 42 条

[1] Photocatalytic Reduction of Graphene Oxide Nanosheets on TiO2 Thin Film for Photoinactivation of Bacteria in Solar Light Irradiation [J].