Age-dependent ultrastructural alterations and biochemical response of rat skeletal muscle after hypoxic or hyperoxic treatments

被引:50
作者
Amicarelli, F
Ragnelli, AM
Aimola, P
Bonfigli, A
Colafarina, S
Di Ilio, C
Miranda, M
机构
[1] Univ Aquila, Dipartimento Biol Base & Appl, I-67100 Laquila, Italy
[2] Ist Super Educ Fis, Chair Gen Biol, I-67100 Laquila, Italy
[3] Univ G DAnnunzio, Dipartimento Sci Biochim, I-66100 Chieti, Italy
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1999年 / 1453卷 / 01期
关键词
skeletal muscle; aging; hypoxia; hyperoxia; antioxidant enzyme; ultrastructure;
D O I
10.1016/S0925-4439(98)00088-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This work deals with the antioxidant enzymatic response and the ultrastructural aspects of the skeletal muscle of young and aged rats kept under hypoxic or hyperoxic normobaric conditions. It is in fact well known that the supply of oxygen at concentrations higher or lower than those occurring under normal conditions can promote oxidative processes that can cause tissue damage. The enzymes investigated were both those directly involved in reactive oxygen species (ROS) scavenging (superoxide dismutase, catalase and selenium-dependent glutathione peroxidase), and those challenged with the detoxication of cytotoxic compounds produced by the action of ROS on biological molecules (glutathione transferase, glyoxalase I, glutathione reductase), in order to obtain a comparative view of the defence strategies used with respect to aging. Our results support the hypothesis that one of the major contributors to the aging process is the oxidative damage produced at least in part by an impairment of the antioxidant enzymatic system. This makes the aged organism particularly susceptible to oxidative stress injury and to the related degenerative diseases, especially in those tissues with high demand for oxidative metabolism. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:105 / 114
页数:10
相关论文
共 35 条
[21]   AGING AND RESPIRATORY MUSCLE METABOLIC PLASTICITY - EFFECTS OF ENDURANCE TRAINING [J].
POWERS, SK ;
LAWLER, J ;
CRISWELL, D ;
LIEU, FK ;
MARTIN, D .
JOURNAL OF APPLIED PHYSIOLOGY, 1992, 72 (03) :1068-1073
[22]  
REDDY KV, 1995, ARCH BIOCHEM BIOPHYS, V323, P6
[23]  
REDDY VR, 1992, BIOCHEM INT, V26, P863
[24]   VITAMIN-E INHIBITS PROTEIN OXIDATION IN SKELETAL-MUSCLE OF RESTING AND EXERCISED RATS [J].
REZNICK, AZ ;
WITT, E ;
MATSUMOTO, M ;
PACKER, L .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 189 (02) :801-806
[25]   CURRENT STATUS OF ANTIOXIDANT THERAPY [J].
RICEEVANS, CA ;
DIPLOCK, AT .
FREE RADICAL BIOLOGY AND MEDICINE, 1993, 15 (01) :77-96
[26]   Lipid peroxidation, antioxidant protection and aging [J].
Rikans, LE ;
Hornbrook, KR .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1997, 1362 (2-3) :116-127
[27]  
Schreck R, 1991, Trends Cell Biol, V1, P39, DOI 10.1016/0962-8924(91)90072-H
[28]   OXIDATIVE DAMAGE AND MITOCHONDRIAL DECAY IN AGING [J].
SHIGENAGA, MK ;
HAGEN, TM ;
AMES, BN .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :10771-10778
[29]   AGING, CYTOCHROME-OXIDASE ACTIVITY, AND HYDROGEN-PEROXIDE RELEASE BY MITOCHONDRIA [J].
SOHAL, RS .
FREE RADICAL BIOLOGY AND MEDICINE, 1993, 14 (06) :583-588
[30]   HYDROGEN-PEROXIDE RELEASE BY MITOCHONDRIA INCREASES DURING AGING [J].
SOHAL, RS ;
SOHAL, BH .
MECHANISMS OF AGEING AND DEVELOPMENT, 1991, 57 (02) :187-202