Exploring the properties of thermostable Clostridium thermocellum cellulase CelE for the purpose of its expression in plants

被引：5

作者：

Abdeev, RM ^{[1
]}

Goldenkova, IV ^{[1
]}

Musiychuk, KA ^{[1
]}

Piruzian, ES ^{[1
]}

机构：

[1] Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119991, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2001年 / 66卷 / 07期

基金：

俄罗斯基础研究基金会;

关键词：

thermostable cellulase (beta-1,4-glucanase); Clostridium thermocellum; plant cellulases;

D O I：

10.1023/A:1010277115744

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The main properties(pH and temperature range, stability, substrate specificity) of the modified cellulase CelE (endo-beta -1,4-glucanase) from Clostridium thermocellum have been analyzed with the goal of its expression in plants. The modified enzyme is similar to plant cellulases. Deletions in the N-terminus of the enzyme do not affect its biochemical properties. Based on the present investigation, we conclude that the modified beta -1,4-glucanase CelEM1,when expressed in plants, will be a good model to study the role of cellulases in plants.

引用

页码：808 / 813

页数：6

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