The polycomb-group homolog Bmi-1 is a regulator of murine Hox gene expression

被引:118
作者
vanderLugt, NMT
Alkema, M
Berns, A
Deschamps, J
机构
[1] NETHERLANDS INST DEV BIOL,HUBRECHT LAB,NL-3584 CT UTRECHT,NETHERLANDS
[2] NETHERLANDS CANC INST,DIV MOL GENET,NL-1066 CX AMSTERDAM,NETHERLANDS
关键词
Bmi-1; polycomb-group; hox; mel-18; patterning;
D O I
10.1016/S0925-4773(96)00570-9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Drosophila homeotic genes and vertebrate Hox genes are involved in the anteroposterior organization of the developing embryo. In Drosophila, the Polycomb- and trithorax-group genes are required to maintain the homeotic genes throughout development in the repressed or activated state, respectively. The murine Bmi-1 proto-oncogene was shown to exhibit homology to the Polycomb-group gene Posterior sex combs. Mice lacking the Bmi-1 gene revealed posterior transformations along the axial skeleton, whereas transgenic mice overexpressing Bmi-1 display anterior transformations. We have analysed the expression patterns of several Hox genes by RNA in situ hybridization on serial sections of 11.5- and 12.5-day Bmi-1 null mutant embryos. Furthermore, we have analysed the expression of a Hoxc-8/LacZ fusion gene in younger embryos. Our analyses show that Bmi-l is involved in the repression of a subset of Hox genes from different clusters from at least day 9.5 onwards. We discuss the possibility that members of the murine Polycomb-group can form multimeric protein complexes of different compositions with varying affinity or specificity for different subsets of Hox genes.
引用
收藏
页码:153 / 164
页数:12
相关论文
共 57 条
  • [21] LAWSON KA, 1991, DEVELOPMENT, V113, P891
  • [22] TARGETED REPLACEMENT OF THE HOMEOBOX GENE HOX-3.1 BY THE ESCHERICHIA-COLI LACZ IN MOUSE CHIMERIC EMBRYOS
    LEMOUELLIC, H
    LALLEMAND, Y
    BRULET, P
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) : 4712 - 4716
  • [23] HOMEOSIS IN THE MOUSE INDUCED BY A NULL MUTATION IN THE HOX-3.1 GENE
    LEMOUELLIC, H
    LALLEMAND, Y
    BRULET, P
    [J]. CELL, 1992, 69 (02) : 251 - 264
  • [24] GENE COMPLEX CONTROLLING SEGMENTATION IN DROSOPHILA
    LEWIS, EB
    [J]. NATURE, 1978, 276 (5688) : 565 - 570
  • [25] LOCKE J, 1988, GENETICS, V120, P181
  • [26] A CONSERVED RETINOIC ACID RESPONSE ELEMENT REQUIRED FOR EARLY EXPRESSION OF THE HOMEOBOX GENE HOXB-1
    MARSHALL, H
    STUDER, M
    POPPERL, H
    APARICIO, S
    KUROIWA, A
    BRENNER, S
    KRUMLAUF, R
    [J]. NATURE, 1994, 370 (6490) : 567 - 571
  • [27] MARTIN EC, 1993, DEVELOPMENT, V117, P641
  • [28] HOMEOBOX GENES AND AXIAL PATTERNING
    MCGINNIS, W
    KRUMLAUF, R
    [J]. CELL, 1992, 68 (02) : 283 - 302
  • [29] A MOUSE HOMEOBOX CONTAINING GENE ON CHROMOSOME-11 - SEQUENCE AND TISSUE-SPECIFIC EXPRESSION
    MEIJLINK, F
    DELAAF, R
    VERRIJZER, P
    DESTREE, O
    KROEZEN, V
    HILKENS, J
    DESCHAMPS, J
    [J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (17) : 6773 - 6786
  • [30] TRANSCRIPTIONAL SILENCING BY THE POLYCOMB PROTEIN IN DROSOPHILA EMBRYOS
    MULLER, J
    [J]. EMBO JOURNAL, 1995, 14 (06) : 1209 - 1220