Following aptamer-ricin specific binding by single molecule recognition and force spectroscopy measurements

被引：28

作者：

Wang, Bin ^{[1
,2
]}

Guo, Cunlan ^{[1
,2
]}

Chen, Guojun ^{[1
,2
]}

Park, Bosoon ^{[3
]}

Xu, Bingqian ^{[1
,2
]}

机构：

[1] Univ Georgia, Single Mol Study Lab, Fac Engn, Athens, GA 30602 USA

[2] Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA

[3] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 11期

基金：

美国国家科学基金会;

关键词：

IN-VITRO SELECTION; DNA; DIAGNOSTICS; MICROSCOPY; HADDOCK; LIGANDS; DOCKING;

D O I：

10.1039/c1cc15644f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Single molecule recognition imaging and dynamic force spectroscopy (DFS) analysis showed strong binding affinity between an aptamer and ricin, which was comparable with antibody-ricin interaction. Molecular simulation showed a ricin binding conformation with aptamers and gave different ricin conformations immobilizing on substrates that were consistent with AFM images.

引用

页码：1644 / 1646

页数：3

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