Single-molecule biophysics: at the interface of biology, physics and chemistry

被引：203

作者：

Deniz, Ashok A. ^{[1
]}

Mukhopadhyay, Samrat ^{[1
]}

Lemke, Edward A. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE ROYAL SOCIETY INTERFACE | 2008年 / 5卷 / 18期

关键词：

single-molecule; fluorescence; force; FRET; tracking; AFM; optical tweezers;

D O I：

10.1098/rsif.2007.1021

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Single-molecule methods have matured into powerful and popular tools to probe the complex behaviour of biological molecules, due to their unique abilities to probe molecular structure, dynamics and function, unhindered by the averaging inherent in ensemble experiments. This review presents an overview of the burgeoning field of single-molecule biophysics, discussing key highlights and selected examples from its genesis to our projections for its future. Following brief introductions to a few popular single-molecule fluorescence and manipulation methods, we discuss novel insights gained from single-molecule studies in key biological areas ranging from biological folding to experiments performed in vivo.

引用

页码：15 / 45

页数：31

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