AFM imaging of protein movements: Histone H2A-H2B release during nucleosome remodeling

被引：36

作者：

Bash, R.

Wang, H.

Anderson, C.

Yodh, J.

Hager, G.

Lindsay, S. M. ^{[1
]}

Lohr, D.

机构：

[1] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA

[2] Midwestern Univ, Arizona Coll Osteopath Med, Div Basic Sci, Glendale, AZ 85308 USA

[3] NCI, Lab Receptor Biol & Gene Express, Bethesda, MD USA

[4] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

[5] Arizona State Univ, Dept Phys & Astron, Tempe, AZ 85287 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 19期

关键词：

chromatin; nucleosome; remodeling; Swi-Snf; histones; SPM; AFM;

D O I：

10.1016/j.febslet.2006.06.101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Being able to follow assembly/disassembly reactions of biomolecular complexes directly at the single molecule level would be very useful. Here, we use an AFM technique that can simultaneously obtain topographic images and identify the locations of a specific type of protein within those images to monitor the histone H2A component of nucleosomes acted on by human Swi-Snf, an ATP-dependent nucleosome remodeling complex. Activation of remodeling results in significant H2A release from nucleosomes, based on recognition imaging and nucleosome height changes, and changes in the recognition patterns of H2A associated directly with hSwi-Snf complexes. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4757 / 4761

页数：5

共 20 条

[1] Nucleosomal arrays can be salt-reconstituted on a single-copy MMTV promoter DNA template: Their properties differ in several ways from those of comparable 5S concatameric arrays [J].