Arabidopsis thaliana expresses a second functional phytochelatin synthase

被引:81
作者
Cazalé, AC [1 ]
Clemens, S [1 ]
机构
[1] Leibniz Inst Plant Biochem, D-06120 Halle Saale, Germany
关键词
heavy metal tolerance; metal chelator; metal homeostasis;
D O I
10.1016/S0014-5793(01)02976-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phytochelatins represent a major detoxifying pathway for heavy metals in plants and many other organisms. The Arabidopsis thaliana CAD1 (= AtPCS1) gene encodes a phytochelatin synthase and cad1 mutants are phytochelatin deficient and cadmium hypersensitive. The Arabidopsis genome contains a highly homologous gene, AtPCS2, of which expression and function were studied in order to understand the apparent non-redundancy of the two genes. Low constitutive AtPCS2 expression is detected in all plant organs analyzed. The AtPCS2 gene encodes a functional phytochelatin synthase as shown by expression in Saccharomyces cerevisiae and the complementation of a Schizosaccharomyces pombe phytochelatin synthase knockout strain. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:215 / 219
页数:5
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