Phaseolus vulgaris seed-borne endophytic community with novel bacterial species such as Rhizobium endophyticum sp. nov.

被引:125
作者
Lopez-Lopez, Aline [1 ]
Rogel, Marco A. [1 ]
Ormeno-Orrillo, Ernesto [1 ]
Martinez-Romero, Julio [1 ]
Martinez-Romero, Esperanza [1 ]
机构
[1] UNAM, Ctr Ciencias Genom, Cuernavaca, Morelos, Mexico
关键词
Legumes; Methylobacterium; Plant symbiosis; Mesoamerican beans; Staphylococcus; Sphingomonas; NITROGEN-FIXING NODULES; PHYTATE UTILIZATION; PLANT; DIVERSITY; IDENTIFICATION; RHIZOSPHERE; PROMOTION; TROPICI; BEANS; SPP;
D O I
10.1016/j.syapm.2010.07.005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The bacterial endophytic community present in different Phaseolus vulgaris (bean) cultivars was analyzed by 16S ribosomal RNA gene sequences of cultured isolates derived from surface disinfected roots and immature seeds. Isolated endophytes from tissue-macerates belonged to over 50 species in 24 different genera and some isolates from Acinetobacter, Bacillus, Enterococcus, Nocardioides, Paracoccus, Phyllobacterium, and Sphingomonas seem to correspond to new lineages. Phytate solubilizing bacteria were identified among Acinetobacter, Bacillus and Streptomyces bean isolates, phytate is the most abundant reserve of phosphorus in bean and in other seeds. Endophytic rhizobia were not capable of forming nodules. A novel rhizobial species Rhizobium endophyticum was recognized on the basis of DNA-DNA hybridization, sequence of 16S rRNA, recA, rpoB, atpD, dnaK genes, plasmid profiles, and phenotypic characteristics. R. endophyticum is capable of solubilizing phytate, the type strain is CCGE2052 (ATCC BAA-2116; HAMBI 3153) that became fully symbiotic by acquiring the R. tropici CFN299 symbiotic plasmid. (C) 2010 Elsevier GmbH. All rights reserved.
引用
收藏
页码:322 / 327
页数:6
相关论文
共 43 条
[1]   Quantitative trait loci for root architecture traits correlated with phosphorus acquisition in common bean [J].
Beebe, SE ;
Rojas-Pierce, M ;
Yan, XL ;
Blair, MW ;
Pedraza, F ;
Muñoz, F ;
Tohme, J ;
Lynch, JP .
CROP SCIENCE, 2006, 46 (01) :413-423
[2]  
Coelho Cileide Maria Medeiros, 2002, Braz. J. Plant Physiol., V14, P51, DOI 10.1590/S1677-04202002000100007
[3]   High incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in Uzbekistan [J].
Egamberdieva, Dilfuza ;
Kamilova, Faina ;
Validov, Shamil ;
Gafurova, Laziza ;
Kucharova, Zulfiya ;
Lugtenberg, Ben .
ENVIRONMENTAL MICROBIOLOGY, 2008, 10 (01) :1-9
[4]   Natural incidence of endophytic bacteria in pea cultivars under field conditions [J].
Elvira-Recuenco, M ;
van Vuurde, JWL .
CANADIAN JOURNAL OF MICROBIOLOGY, 2000, 46 (11) :1036-1041
[5]   THE INFECTION OF CLOVER ROOT HAIRS BY NODULE BACTERIA STUDIED BY A SIMPLE GLASS SLIDE TECHNIQUE [J].
FAHRAEUS, G .
JOURNAL OF GENERAL MICROBIOLOGY, 1957, 16 (02) :374-&
[6]   Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia [J].
Gaunt, MW ;
Turner, SL ;
Rigottier-Gois, L ;
Lloyd-Macgilp, SA ;
Young, JPW .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :2037-2048
[7]   Multiresistance genes of Rhizobium etli CFN42 [J].
González-Pasayo, R ;
Martínez-Romero, E .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (05) :572-577
[8]   Bacterial endophytes in agricultural crops [J].
Hallmann, J ;
QuadtHallmann, A ;
Mahaffee, WF ;
Kloepper, JW .
CANADIAN JOURNAL OF MICROBIOLOGY, 1997, 43 (10) :895-914
[9]   Properties of bacterial endophytes and their proposed role in plant growth [J].
Hardoim, Pablo R. ;
van Overbeek, Leo S. ;
van Elsas, Jan Dirk .
TRENDS IN MICROBIOLOGY, 2008, 16 (10) :463-471
[10]   PPFMS AND OTHER COVERT CONTAMINANTS - IS THERE MORE TO PLANT PHYSIOLOGY THAN JUST PLANT [J].
HOLLAND, MA ;
POLACCO, JC .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1994, 45 :197-209