A Versatile DNA Nanochip for Direct Analysis of DNA Base-Excision Repair

被引：91

作者：

Endo, Masayuki ^{[1
,3
]}

Katsuda, Yousuke ^{[2
]}

Hidaka, Kumi ^{[2
]}

Sugiyama, Hiroshi ^{[1
,2
,3
]}

机构：

[1] Kyoto Univ, Inst Integrated Cell Mat Sci iCeMS, Sakyo Ku, Kyoto 6068501, Japan

[2] Kyoto Univ, Dept Chem, Grad Sch Sci, Sakyo Ku, Kyoto 6068502, Japan

[3] Japan Sci & Technol Corp JST, Chiyoda Ku, Tokyo 1020075, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 49期

关键词：

DNA damage; enzymes; nanostructures; scanning probe microscopy; single-molecule studies; PYRIMIDINE DIMER; STRUCTURAL BASIS; 8-OXOGUANINE; RECOGNITION; DAMAGE;

D O I：

10.1002/anie.201003604

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Repair kit: The single DNA-repair enzymes 8-oxoguanine glycosylase and T4 pyrimidine dimer glycosylase were analyzed by a nanoscale DNA chip containing two double-stranded DNA molecules (see picture). Dynamic movement of the enzymes and the single DNA-repair reaction on the DNA nanochip was visualized by fast-scanning atomic force microscopy. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9412 / 9416

页数：5

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