Factors affecting functional outcome after autologous skeletal myoblast transplantation

被引:159
作者
Pouzet, B
Vilquin, JT
Hagège, AA
Scorsin, M
Messas, E
Fiszman, M
Schwartz, K
Menasché, P
机构
[1] Grp Hosp Pitie Salpetriere, Inst Myol, INSERM, U523,Hop Bichat,Dept Cardiovasc Surg, F-75634 Paris, France
[2] Hop Boucicault, Dept Cardiol, Paris, France
关键词
D O I
10.1016/S0003-4975(00)01785-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background. This study assessed the extent to which the initial degree of functional impairment and the number of injected cells may influence the functional improvement provided by autologous skeletal myoblast transplantation into infarcted myocardium. Methods. One week after left coronary artery ligation, 44 rats received into the infarcted scar, autologous skeletal myoblasts expanded in vitro for 7 days (mean, 3.5 x 10(6), n = 21), or culture medium alone (controls, n 23). Left ventricular function was assessed by two-dimensional echocardiography. Results; When transplanted hearts were stratified according to their baseline ejection fraction, a significant improvement occurred at 2 months in the less than 25% (from 21.4% to 37%), 25% to 35% (from 29% to 43.8%), and in the 35% to 40% (from 37.2% to 41.7%) groups, compared to controls (p = 0.048, 0.0057, and 0.034, respectively), but not in the more than 40% stratum. A significant linear relationship was found between the improvement in ejection fraction and the number of injected myoblasts, both at 1 and 2 months after transplantation (p < 0.0001). Conclusions. Autologous myoblast transplantation is functionally effective over a wide range of postinfarct ejection fractions, including in the sickest hearts provided that they are injected with a sufficiently high number of cells. (C) 2001 by The Society of Thoracic Surgeons.
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收藏
页码:844 / 850
页数:7
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