Evaluation of tissue preparation for in vitro study of hepatic long-chain fatty acid metabolism

被引：3

作者：

Andersen, JB ^{[1
]}

Larsen, T ^{[1
]}

Ingvartsen, L ^{[1
]}

机构：

[1] Danish Inst Agr Sci, Res Ctr Foulum, Dept Anim Hlth & Welf, DK-8830 Tjele, Denmark

来源：

ACTA AGRICULTURAE SCANDINAVICA SECTION A-ANIMAL SCIENCE | 2001年 / 51卷 / 01期

关键词：

cow; fatty acid oxidation; in vitro; ketogenesis; liver;

D O I：

10.1080/090647001300004781

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The purpose of the present study was to compare the effect of different tissue preparations on hepatic long-chain fatty, acid oxidation and ketogenesis in a tissue culture system. The liver tissue was prepared using a Stadie-Riggs hand microtome (SR), a motorized tissue slicer (Krumdieck tissue slicer, KTS) or a small needle biopsy (G14; 14G x 17 mm notch needle biopsy or G16; 16G x 17 mm needle biopsy). The liver tissue was obtained from slaughtered lactating dairy cows. The CO, and ketone body production were significantly affected by tissue preparation in the positive order KTS > SR > G14 > G16. The variability of the in vitro results was slightly affected by tissue preparation, being highest for the SR method. The G14 small needle biopsy had a sufficiently good biological response, and presumably small needle biopsy can minimize health risks compared with taking bigger liver biopsies on living animals.

引用

页码：47 / 52

页数：6

共 29 条

[11] A TECHNIQUE FOR ISOLATION OF BOVINE HEPATOCYTES
FORSELL, JH
JESSE, BW
SHULL, LR
[J]. JOURNAL OF ANIMAL SCIENCE, 1985, 60 (06) : 1597 - 1609
[12] FRESHNEY RI, 1994, CULTURE ANIMALS CELL
[13] Cytochrome P450 isoenzyme activities in cultured rat and mouse liver slices
Gokhale, MS
Bunton, TE
Zurlo, J
Yager, JD
[J]. XENOBIOTICA, 1997, 27 (04) : 341 - 355
[14] Nutrition during the dry period and hepatic lipid metabolism of periparturient dairy cows
Grum, DE
Drackley, JK
Younker, RS
LaCount, DW
Veenhuizen, JJ
[J]. JOURNAL OF DAIRY SCIENCE, 1996, 79 (10) : 1850 - 1864
[15] PEROXISOMAL BETA-OXIDATION OF FATTY-ACIDS IN BOVINE AND RAT-LIVER
GRUM, DE
HANSEN, LR
DRACKLEY, JK
[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1994, 109 (2-3): : 281 - 292
[16] Production, digestion, and hepatic lipid metabolism of dairy cows fed increased energy from fat or concentrate
Grum, DE
Drackley, JK
Hansen, LR
Cremin, JD
[J]. JOURNAL OF DAIRY SCIENCE, 1996, 79 (10) : 1836 - 1849
[17] Intestinal absorption, blood transport and hepatic and muscle metabolism of fatty acids in preruminant and ruminant animals
Hocquette, JF
Bauchart, D
[J]. REPRODUCTION NUTRITION DEVELOPMENT, 1999, 39 (01): : 27 - 48
[18] HUGHES JP, 1962, AM J VET RES, V23, P1111
[19] CONTROL OF BOVINE HEPATIC FATTY-ACID OXIDATION
JESSE, BW
EMERY, RS
THOMAS, JW
[J]. JOURNAL OF DAIRY SCIENCE, 1986, 69 (09) : 2290 - 2297
[20] ASPECTS OF THE REGULATION OF LONG-CHAIN FATTY-ACID OXIDATION IN BOVINE LIVER
JESSE, BW
EMERY, RS
THOMAS, JW
[J]. JOURNAL OF DAIRY SCIENCE, 1986, 69 (09) : 2298 - 2303

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