YopT, a new Yersinia Yop effector protein, affects the cytoskeleton of host cells

被引:196
作者
Iriarte, M [1 ]
Cornelis, GR [1 ]
机构
[1] Univ Catholique Louvain, Fac Med, Microbial Pathogenesis Unit, Christian de Duve Inst Cellular Pathol, B-1200 Brussels, Belgium
关键词
D O I
10.1046/j.1365-2958.1998.00992.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Extracellular Yersinia disarm the immune system of their host by injecting effector Yop proteins into the cytosol of target cells. Five effecters have been described: YopE, YopH, YpkA/YopO, YopP and YopM. Delivery of these effecters by Yersinia adhering at the cell surface requires other Yops (translocators) including YopB. Effector and translocator Yops are secreted by the type III Ysc secretion apparatus, and some Yops also need a specific cytosolic chaperone, called Syc. In this paper, we describe a new Yop, which we have called YopT (35.5 kDa). Its secretion required an intact Ysc apparatus and SycT (15.0 kDa, pi 4.4), a new chaperone resembling SycE. Infection of macrophages with a Yersinia, producing a hybrid YopT-adenylate cyclase, led to the accumulation of intracellular cAMP, indicating that YopT is delivered into the cytosol of eukaryotic cells. Infection of HeLa cells with a mutant strain devoid of the five known Yop effecters (Delta HOPEM strain) but producing YopT resulted in the alteration of the cell cytoskeleton and the disruption of the actin filament structure. This cytotoxic effect was caused by YopT and dependent on YopB. YopT is thus a new effector Yop and a new bacterial toxin affecting the cytoskeleton of eukaryocells.
引用
收藏
页码:915 / 929
页数:15
相关论文
共 69 条
[11]   Role of YopP in suppression of tumor necrosis factor alpha release by macrophages during Yersinia infection [J].
Boland, A ;
Cornelis, GR .
INFECTION AND IMMUNITY, 1998, 66 (05) :1878-1884
[12]   Heparin interferes with translocation of Yop proteins into HeLa cells and binds to LcrG, a regulatory component of the Yersinia Yop apparatus [J].
Boyd, AP ;
Sory, MP ;
Iriarte, M ;
Cornelis, GR .
MOLECULAR MICROBIOLOGY, 1998, 27 (02) :425-436
[13]   Two independent type III secretion mechanisms for YopE in Yersinia enterocolitica [J].
Cheng, LW ;
Anderson, DM ;
Schneewind, O .
MOLECULAR MICROBIOLOGY, 1997, 24 (04) :757-765
[14]   THE PYV PLASMID OF YERSINIA ENCODES A LIPOPROTEIN, YLPA, RELATED TO TRAT [J].
CHINA, B ;
MICHIELS, T ;
CORNELIS, GR .
MOLECULAR MICROBIOLOGY, 1990, 4 (09) :1585-1593
[15]   2 LINKED GENES FOR OUTER-MEMBRANE PROTEINS ARE ABSENT IN 4 NONDISEASE STRAINS OF HAEMOPHILUS-SOMNUS [J].
COLE, SP ;
GUINEY, DG ;
CORBEIL, LB .
MOLECULAR MICROBIOLOGY, 1992, 6 (14) :1895-1902
[16]   MOLECULAR ANALYSIS OF A GENE ENCODING A SERUM-RESISTANCE-ASSOCIATED 76-KDA SURFACE-ANTIGEN OF HAEMOPHILUS-SOMNUS [J].
COLE, SP ;
GUINEY, DG ;
CORBEIL, LB .
JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 :2135-2143
[17]   Haemophilus somnus immunoglobulin binding proteins and surface fibrils [J].
Corbeil, LB ;
BastidaCorcuera, FD ;
Beveridge, TJ .
INFECTION AND IMMUNITY, 1997, 65 (10) :4250-4257
[18]  
CORNELIS G, 1975, J GEN MICROBIOL, V87, P285, DOI 10.1099/00221287-87-2-285
[19]   HOMOLOGY BETWEEN VIRF, THE TRANSCRIPTIONAL ACTIVATOR OF THE YERSINIA VIRULENCE REGULON, AND ARAC, THE ESCHERICHIA-COLI ARABINOSE OPERON REGULATOR [J].
CORNELIS, G ;
SLUITERS, C ;
DEROUVROIT, CL ;
MICHIELS, T .
JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :254-262
[20]   BETA-LACTAMASES FROM YERSINIA-ENTEROCOLITICA [J].
CORNELIS, G ;
ABRAHAM, EP .
JOURNAL OF GENERAL MICROBIOLOGY, 1975, 87 (APR) :273-284