Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing

被引：213

作者：

Cheng, Adrian ^{[1
,2
,3
]}

Goncalves, J. Tiago

Golshani, Peyman ^{[1
]}

Arisaka, Katsushi ^{[2
,4
]}

Portera-Cailliau, Carlos ^{[1
,3
]}

机构：

[1] Univ Calif Los Angeles, Dept Neurol, David Geffen Sch Med, Los Angeles, CA 90024 USA

[2] Univ Calif Los Angeles, Dept Phys & Astron, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, Dept Neurobiol, David Geffen Sch Med, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, California NanoSyst Inst, Los Angeles, CA 90024 USA

来源：

NATURE METHODS | 2011年 / 8卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

HIGH-SPEED; MICROSCOPY; NETWORK; PHOTODAMAGE; DYNAMICS; NEURONS;

D O I：

10.1038/NMETH.1552

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In vivo two-photon calcium imaging would benefit from the use of multiple excitation beams to increase scanning speed, signal-to-noise ratio and field of view or to image different axial planes simultaneously. Using spatiotemporal multiplexing we circumvented light-scattering ambiguity inherent to deep-tissue multifocal two-photon microscopy. We demonstrate calcium imaging at multiple axial planes in the intact mouse brain to monitor network activity of ensembles of cortical neurons in three spatial dimensions.

引用

页码：139 / U58

页数：6

共 29 条

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Simultaneous imaging of multiple focal planes using a two-photon scanning microscope [J].