A Combined Transcriptomics and Lipidomics Analysis of Subcutaneous, Epididymal and Mesenteric Adipose Tissue Reveals Marked Functional Differences

被引：82

作者：

Caesar, Robert ^{[1
,2
]}

Manieri, Monia ^{[3
]}

Kelder, Thomas ^{[4
]}

Boekschoten, Mark ^{[5
,6
]}

Evelo, Chris ^{[4
]}

Mueller, Michael ^{[5
,6
]}

Kooistra, Teake ^{[7
]}

Cinti, Saverio ^{[3
]}

Kleemann, Robert ^{[7
]}

Drevon, Christian A. ^{[1
]}

机构：

[1] Univ Oslo, Inst Basic Med Sci, Fac Med, Dept Nutr, Oslo, Norway

[2] Univ Gothenburg, Sahlgrenska Ctr Cardiovasc & Metab Res, Wallenberg Lab, Gothenburg, Sweden

[3] Univ Ancona, Sch Med, Dept Mol Pathol & Innovat Therapies, Ancona, Italy

[4] Maastricht Univ, Dept Bioinformat, BiGCaT, Maastricht, Netherlands

[5] TI Food & Nutr, Nutrigenom Consortium, Wageningen, Netherlands

[6] Wageningen Univ, Div Human Nutr, Nutr Metab & Genom Grp, Wageningen, Netherlands

[7] TNO Qual Life, BioSci, Leiden, Netherlands

来源：

PLOS ONE | 2010年 / 5卷 / 07期

关键词：

ELEMENT-BINDING PROTEIN; MESSENGER-RNA EXPRESSION; FATTY-ACID-COMPOSITION; GENE-EXPRESSION; LYMPHOID-CELLS; INSULIN SENSITIVITY; HUMAN PREADIPOCYTES; GLUCOSE-METABOLISM; OBESE SUBJECTS; IN-VITRO;

D O I：

10.1371/journal.pone.0011525

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Depot-dependent differences in adipose tissue physiology may reflect specialized functions and local interactions between adipocytes and surrounding tissues. We combined time-resolved microarray analyses of mesenteric- (MWAT), subcutaneous- (SWAT) and epididymal adipose tissue (EWAT) during high-fat feeding of male transgenic ApoE3Leiden mice with histology, targeted lipidomics and biochemical analyses of metabolic pathways to identify differentially regulated processes and site-specific functions. EWAT was found to exhibit physiological zonation. De novo lipogenesis in fat proximal to epididymis was stably low, whereas de novo lipogenesis distal to epididymis and at other locations was down-regulated in response to high-fat diet. The contents of linoleic acid and alpha-linolenic acid in EWAT were increased compared to other depots. Expression of the androgen receptor (Ar) was higher in EWAT than in MWAT and SWAT. We suggest that Ar may mediate depot-dependent differences in de novo lipogenesis rate and propose that accumulation of linoleic acid and alpha-linolenic acid in EWAT is favored by testosterone-mediated inhibition of de novo lipogenesis and may promote further elongation and desaturation of these polyunsaturated fatty acids during spermatogenesis.

引用

页数：14

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