Comparative investigations of the effects of X- and UV-irradiation on lysozyme in the absence or presence of additives

被引：25

作者：

Durchschlag, H

Hefferle, T

Zipper, P

机构：

[1] Univ Regensburg, Inst Biophys & Phys Biochem, D-93040 Regensburg, Germany

[2] Graz Univ, Inst Chem, A-8010 Graz, Austria

来源：

RADIATION PHYSICS AND CHEMISTRY | 2003年 / 67卷 / 3-4期

关键词：

lysozyme; structure and function; radiation damage; additives; modeling;

D O I：

10.1016/S0969-806X(03)00089-6

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The radiation damage of lysozyme has been investigated in aqueous solution, after preceding X- and UV-irradiation in the absence or presence of various additives. Activity measurements and UV absorption and fluorescence spectroscopy turned out to be powerful screening techniques for disclosing the damages (inactivation, aggregation, loss of aromatics) and modifications (protection) provided by many additives. Advanced computer modeling approaches were used to visualize the protein atoms and the water molecules bound preferentially on. the protein surface. Correlations between the position of radiosensitive amino acid residues and occurring radiation damages are discussed. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：479 / 486

页数：8

共 38 条

[1] ON MECHANISM OF RADIATION-INDUCED INACTIVATION OF LYSOZYME IN DILUTE AQUEOUS SOLUTION [J].