Identification of GM-CSF in Paneth cells using single-cell RT-PCR

被引：50

作者：

Fukuzawa, H ^{[1
]}

Sawada, M ^{[1
]}

Kayahara, T ^{[1
]}

Morita-Fujisawa, Y ^{[1
]}

Suzuki, K ^{[1
]}

Seno, H ^{[1
]}

Takaishi, S ^{[1
]}

Chiba, T ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, Sakyo Ku, Kyoto 6068507, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 312卷 / 04期

基金：

日本学术振兴会;

关键词：

Paneth cell; GM-CSF; CD80; CD86; small intestine;

D O I：

10.1016/j.bbrc.2003.11.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Paneth cells, granule-containing cells located at the bottom of the intestinal crypts, have a role in innate mucosal immunity. We identified the exclusive expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in Paneth cells using single-cell reverse transcription-polymerase chain reaction and cDNA array. Cytosolic total RNA was aspirated from single Paneth cells and other villous epithelial cells (non-Paneth cells) of rats using capillary micropipettes. In addition to lysozyme, secretory phospholipase A2, defensin, TNF-alpha, and xanthine dehydrogenase genes, cDNA array analysis revealed that the GM-CSF gene is specifically present in Paneth cells, whereas GM-CSF receptor beta-chain mRNA is expressed in Paneth cells and other epithelial cells. There was intense immunohistochemical staining of GM-CSF in Paneth cells but not in other epithelial cells. Treatment of IEC6 cells with GM-CSF enhanced expression of CD80 and CD86. Thus, GM-CSF in Paneth cells might have an important role in mucosal immunity through increasing the expression of costimulatory molecules in epithelial cells. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：897 / 902

页数：6

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